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Whole Cell Lysates for Western Blotting

Santa Cruz Biotechnology offers whole cell lysates that are provided as Western blot positive controls. Each vial contains 500 µg protein in 200 µl SDS-PAGE Western blotting buffer. Recommended use is 50 µg (20 µl) per lane. Lysates should be stored at -20°C and repeated freezing and thawing should be minimized. Sample vial should be boiled once prior to use. Select lysates, as indicated, are treated with alkaline phosphatase (AP) as phosphorylation depleted negative controls for phosphorylation-induction studies.

SEE ALSO...

PRODUCT NAME CATALOG # Amount Species Citations RATING
3611-RF Whole Cell Lysate sc-2215 500 µg/200 µl r 3
(1)
3T3-L1 Cell Lysate sc-2243 500 µg/200 µl m 3
(1)
A-10 Cell Lysate sc-3806 500 µg/200 µl r 1,607
(2)
A-431 + Calyculin A Cell Lysate sc-2260 500 µg/200 µl h 1
(0)
A-431 Whole Cell Lysate sc-2201 500 µg/200 µl h 11
(1)
A-375 Cell Lysate sc-3811 500 µg/200 µl h 1
(0)
A549 Cell Lysate sc-2413 500 µg/200 µl h 2
(0)
A-673 Cell Lysate sc-2414 500 µg/200 µl h 3
(0)
ACHN Whole Cell Lysate sc-364365 500 µg/200 µl h
(0)
ALL-SIL Whole Cell Lysate sc-364356 500 µg/200 µl h
(0)
AMJ2-C8 Whole Cell Lysate sc-364366 500 µg/200 µl m
(0)
ARPE-19 Whole Cell Lysate sc-364357 500 µg/200 µl h
(1)
AT3B-1 Whole Cell Lysate sc-364372 500 µg/200 µl r
(0)
AtT-20/D16vF2 Whole Cell Lysate sc-364367 500 µg/200 µl m
(0)
BC3H1 Cell Lysate sc-2299 500 µg/200 µl m
(0)
BE (2)-M17 Whole Cell Lysate sc-364358 500 µg/200 µl h
(0)
BJAB Whole Cell Lysate sc-2207 500 µg/200 µl h 7
(1)
BYDP Whole Cell Lysate sc-364368 500 µg/200 µl m
(0)
BT-20 Cell Lysate sc-2223 500 µg/200 µl h 1
(1)
C6 Whole Cell Lysate sc-364373 500 µg/200 µl r 1
(0)
Ca Ski Whole Cell Lysate sc-364360 500 µg/200 µl h
(0)
C32 Whole Cell Lysate sc-2205 500 µg/200 µl h 4
(2)
C3H/10T1/2 Cell Lysate sc-3801 500 µg/200 µl m
(0)
Caco-2 Cell Lysate sc-2262 500 µg/200 µl h
(1)
Caki-1 Cell Lysate sc-2224 500 µg/200 µl h 4
(1)
CCD-1064Sk + PDGF Cell Lysate sc-2264 500 µg/200 µl h 2
(0)
CCD-1064Sk Cell Lysate sc-2263 500 µg/200 µl h 7
(0)
COS Whole Cell Lysate sc-364228 500 µg/200 µl monkey
(0)
CSMLO Whole Cell Lysate sc-364369 500 µg/200 µl m
(0)
Daoy Whole Cell Lysate sc-364381 500 µg/200 µl h
(0)
CCRF-CEM Cell Lysate sc-2225 500 µg/200 µl h
(0)
CCRF-HSB-2 Cell Lysate sc-2265 500 µg/200 µl h 2
(0)
CHO-K1 Cell Lysate sc-3809 500 µg/200 µl hamster
(0)
COLO 320DM Cell Lysate sc-2226 500 µg/200 µl h 11
(0)
CTLL-2 Cell Lysate sc-2242 500 µg/200 µl m 3
(0)
EGY48 Whole Cell Lysate sc-364775 500 µg/200 µl yeast
(0)
Daudi + IL-4 Cell Lysate sc-2267 500 µg/200 µl h 1
(0)
ES-D3 Whole Cell Lysate sc-364776 500 µg/200 µl m
(0)
Daudi Cell Lysate sc-2415 500 µg/200 µl h 2
(0)
GH3 Whole Cell Lysate sc-364777 500 µg/200 µl r
(0)
GM637 Whole Cell Lysate sc-364361 500 µg/200 µl h
(0)
H69AR Whole Cell Lysate sc-364382 500 µg/200 µl h
(0)
DU 145 Cell Lysate sc-2268 500 µg/200 µl h
(0)
ECV304 Cell Lysate sc-2269 500 µg/200 µl h 3
(0)
F9 Cell Lysate sc-2245 500 µg/200 µl m 7
(0)
FHs 173We Cell Lysate sc-2417 500 µg/200 µl h
(0)
H4 Cell Lysate sc-2408 500 µg/200 µl h 1
(0)
HEL 92.1.7 Cell Lysate sc-2270 500 µg/200 µl h 1
(0)
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl h 32
(13)
K-562 Whole Cell Lysate sc-2203 500 µg/200 µl h 12
(1)
KNRK Whole Cell Lysate sc-2214 500 µg/200 µl r 5
(0)
L8 Cell Lysate sc-3807 500 µg/200 µl r
(0)
LNCaP Cell Lysate sc-2231 500 µg/200 µl h 4
(1)
MCF7 + etopside Cell Lysate sc-2281 500 µg/200 µl h 1
(0)
MCF7 Whole Cell Lysate sc-2206 500 µg/200 µl h 10
(0)
MDA-MB-231 Cell Lysate sc-2232 500 µg/200 µl h 3
(1)
MDA-MB-468 Cell Lysate sc-2282 500 µg/200 µl h
(0)
MDCK Cell Lysate sc-2252 500 µg/200 µl canine
(2)
MEG-01 Cell Lysate sc-2283 500 µg/200 µl h
(0)
MES-SA/Dx5 Cell Lysate sc-2284 500 µg/200 µl h 4
(1)
MIA PaCa-2 Cell Lysate sc-2285 500 µg/200 µl h 1
(1)
MM-142 Cell Lysate sc-2246 500 µg/200 µl m 1
(0)
MOLT-4 Cell Lysate sc-2233 500 µg/200 µl h 2
(1)
Hs 181 Tes Whole Cell Lysate sc-364779 500 µg/200 µl h
(0)
Mv 1 Lu Cell Lysate sc-3810 500 µg/200 µl mink
(0)
NAMALWA Cell Lysate sc-2234 500 µg/200 µl h 1
(0)
NIH/3T3 + anisomycin Cell Lysate sc-2247 500 µg/200 µl m
(0)
I-11.15 Whole Cell Lysate sc-364370 500 µg/200 µl m
(0)
NIH/3T3 + heat shock Cell Lysate sc-2217 500 µg/200 µl m 3
(0)
JEG-3 Whole Cell Lysate sc-364255 500 µg/200 µl h
(0)
NIH/3T3 + PDGF Cell Lysate sc-3803 500 µg/200 µl m 1
(0)
NIH/3T3 Whole Cell Lysate sc-2210 500 µg/200 µl m 23
(1)
NIH/3T3 + serum Cell Lysate sc-2248 500 µg/200 µl m
(0)
NIH/3T3 + UV Cell Lysate sc-3804 500 µg/200 µl m
(0)
PC-12 Cell Lysate sc-2250 500 µg/200 µl r 8
(0)
PC-12 + NGF Cell Lysate sc-3808 500 µg/200 µl r 1
(0)
PC-3 Cell Lysate sc-2220 500 µg/200 µl h 3
(0)
Ramos Cell Lysate sc-2216 500 µg/200 µl h 6
(0)
RAW 264.7 + IFN-γ Cell Lysate sc-2259 500 µg/200 µl m
(0)
RAW 264.7 + LPS/PMA Cell Lysate sc-2212 500 µg/200 µl m 9
(2)
RAW 309 Cr.1 Cell Lysate sc-3814 500 µg/200 µl m
(0)
Saos-2 Cell Lysate sc-2235 500 µg/200 µl h 1
(0)
M1 Whole Cell Lysate sc-364782 500 µg/200 µl m
(0)
SH-SY5Y Cell Lysate sc-3812 500 µg/200 µl h 1
(0)
SJRH30 Cell Lysate sc-2287 500 µg/200 µl h 1
(0)
SK-BR-3 Cell Lysate sc-2218 500 µg/200 µl h 3
(0)
SK-LMS-1 Cell Lysate sc-3813 500 µg/200 µl h
(0)
SK-MEL-28 + IFN-α Cell Lysate sc-2290 500 µg/200 µl h
(0)
SK-MEL-28 + IFN-γ Cell Lysate sc-2291 500 µg/200 µl h 2
(0)
SK-MEL-28 Cell Lysate sc-2236 500 µg/200 µl h 1
(0)
MH-S Whole Cell Lysate sc-364785 500 µg/200 µl m
(0)
SK-N-MC Cell Lysate sc-2237 500 µg/200 µl h 3
(0)
mouse LacZ Whole Cell Lysate sc-364371 500 µg/200 µl m
(0)
MTE1D Whole Cell Lysate sc-364918 500 µg/200 µl m
(0)
SK-N-SH + nocodazole Cell Lysate sc-2289 500 µg/200 µl h
(0)
SK-N-SH Cell Lysate sc-2410 500 µg/200 µl h 6
(0)
NCI-H460 Whole Cell Lysate sc-364235 500 µg/200 µl h
(0)
NCI-H929 Whole Cell Lysate sc-364786 500 µg/200 µl h
(0)
Sol8 Cell Lysate sc-2249 500 µg/200 µl m 3
(0)
SW480 Cell Lysate sc-2219 500 µg/200 µl h 1
(0)
T24 Cell Lysate sc-2292 500 µg/200 µl h 1
(1)
T-47D Cell Lysate sc-2293 500 µg/200 µl h 3
(0)
T98G Cell Lysate sc-2294 500 µg/200 µl h 1
(0)
TE671 Cell Lysate sc-2416 500 µg/200 µl h 1
(0)
TF-1 Cell Lysate sc-2412 500 µg/200 µl h
(0)
THP-1 Cell Lysate sc-2238 500 µg/200 µl h 13
(1)
U-2 OS Cell Lysate sc-2295 500 µg/200 µl h
(0)
U-87 MG Cell Lysate sc-2411 500 µg/200 µl h 5
(0)
U-937 + PMA Cell Lysate sc-2296 500 µg/200 µl h
(0)
U-937 Cell Lysate sc-2239 500 µg/200 µl h 8
(0)
U-937 + TNFα Cell Lysate sc-2297 500 µg/200 µl h
(0)
NK-92 Whole Cell Lysate sc-364788 500 µg/200 µl h
(0)
WEHI-231 Whole Cell Lysate sc-2213 500 µg/200 µl m 3
(0)
WEHI-3 Cell Lysate sc-3815 500 µg/200 µl m
(0)
PANC-1 Whole Cell Lysate sc-364380 500 µg/200 µl h
(1)
WR19L Cell Lysate sc-3805 500 µg/200 µl m 1
(0)
Y79 Cell Lysate sc-2240 500 µg/200 µl h 3
(0)
ZR-75-1 Cell Lysate sc-2241 500 µg/200 µl h 3
(0)
A-431 + AP Cell Lysate sc-24651 500 µg/200 µl h
(0)
Raji Whole Cell Lysate sc-364236 500 µg/200 µl h 1
(0)
A-431 + pervanadate Cell Lysate sc-24654 500 µg/200 µl h
(0)
A-431 + UV Cell Lysate sc-24656 500 µg/200 µl h
(0)
RBL-1 Whole Cell Lysate sc-364790 500 µg/200 µl r
(0)
RD Whole Cell Lysate sc-364791 500 µg/200 µl h
(0)
RKO Whole Cell Lysate sc-364793 500 µg/200 µl h
(0)
RT-4 Whole Cell Lysate sc-364257 500 µg/200 µl h
(0)
SCC-4 Whole Cell Lysate sc-364363 500 µg/200 µl h
(0)
SHP-77 Whole Cell Lysate sc-364258 500 µg/200 µl h
(0)
AN3 CA Cell Lysate sc-24662 500 µg/200 µl h
(0)
SK-MEL-24 Whole Cell Lysate sc-364259 500 µg/200 µl h
(0)
SK-OV-3 Whole Cell Lysate sc-364229 500 µg/200 µl h
(0)
SP2/0 Whole Cell Lysate sc-364795 500 µg/200 µl m
(0)
EB1 Cell Lysate sc-24668 500 µg/200 µl
(0)
T84 Whole Cell Lysate sc-364797 500 µg/200 µl h
(0)
U-698-M Whole Cell Lysate sc-364799 500 µg/200 µl h
(0)
ES-2 Cell Lysate sc-24674 500 µg/200 µl h 1
(0)
U266 Whole Cell Lysate sc-364800 500 µg/200 µl h
(0)
WI-38 Whole Cell Lysate sc-364260 500 µg/200 µl h
(0)
HCT-8 Cell Lysate sc-24675 500 µg/200 µl h
(0)
XP12RO Whole Cell Lysate sc-364364 500 µg/200 µl h
(0)
HeLa + anisomycin Cell Lysate sc-24676 500 µg/200 µl h
(0)
HeLa + CoCl2 Cell Lysate sc-24679 500 µg/200 µl h 14
(0)
HeLa + hydrogen peroxide Cell Lysate sc-24681 500 µg/200 µl h 1
(0)
HeLa + hydroxyurea Cell Lysate sc-24682 500 µg/200 µl h
(0)
HeLa + IFN-α + Calyculin A Cell Lysate sc-24684 500 µg/200 µl h
(0)
HeLa + IL-4 Cell Lysate sc-24686 500 µg/200 µl h
(0)
HeLa + IL-6 Cell Lysate sc-24687 500 µg/200 µl h
(0)
HeLa + pervanadate Cell Lysate sc-24688 500 µg/200 µl h
(0)
HeLa + serum-starved Cell Lysate sc-24693 500 µg/200 µl h
(0)
HeLa + serum-starved + PMA Cell Lysate sc-24695 500 µg/200 µl h
(0)
HeLa + sodium butyrate Cell Lysate sc-24696 500 µg/200 µl h
(0)
HeLa + staurosporine Cell Lysate sc-24698 500 µg/200 µl h
(0)
Hep G2 + TGFβ Cell Lysate sc-24702 500 µg/200 µl h
(0)
HL-60 + DMSO Cell Lysate sc-24703 500 µg/200 µl h
(0)
HL-60 + LPS Cell Lysate sc-24704 500 µg/200 µl h
(0)
HL-60 + PMA Cell Lysate sc-24705 500 µg/200 µl h
(0)
HUV-EC-C + VEGF Cell Lysate sc-24709 500 µg/200 µl h
(1)
Jurkat + AP Cell Lysate sc-24712 500 µg/200 µl h
(0)
Jurkat + camptothecin Cell Lysate sc-24713 500 µg/200 µl h
(0)
Jurkat + hydrogen peroxide Cell Lysate sc-24714 500 µg/200 µl h
(0)
Jurkat + pervanadate Cell Lysate sc-24716 500 µg/200 µl h
(0)
Jurkat + PMA Cell Lysate sc-24718 500 µg/200 µl h
(0)
Jurkat + staurosporine Cell Lysate sc-24719 500 µg/200 µl h 1
(0)
K-562 + anisomycin Cell Lysate sc-24720 500 µg/200 µl h
(0)
K-562 + TNFα Cell Lysate sc-24723 500 µg/200 µl h
(0)
L6 + IGF Cell Lysate sc-24727 500 µg/200 µl r 2
(0)
L428 Cell Lysate sc-24728 500 µg/200 µl h
(0)
L929 Cell Lysate sc-24729 500 µg/200 µl m
(0)
MCF7 + EGF + β-estradiol Cell Lysate sc-24730 500 µg/200 µl h
(0)
MCF7 + insulin Cell Lysate sc-24733 500 µg/200 µl h
(0)
Mv 1 Lu + TGF Cell Lysate sc-24737 500 µg/200 µl mink
(0)
NIH/3T3 + forskolin Cell Lysate sc-24741 500 µg/200 µl m 1
(0)
NIH/3T3 + PMA Cell Lysate sc-24748 500 µg/200 µl m 1
(0)
Quiescent NIH/3T3 Cell Lysate sc-24752 500 µg/200 µl m
(0)
PC-12 + PMA Cell Lysate sc-24757 500 µg/200 µl r
(0)
Ramos + IL-4 Cell Lysate sc-24762 500 µg/200 µl h
(0)
RAW 264.7 + LPS/IFN-γ Cell Lysate sc-24767 500 µg/200 µl m 5
(1)
RAW 264.7 + TNFα Cell Lysate sc-24768 500 µg/200 µl m
(0)
RAW 264.7 + UV Cell Lysate sc-24769 500 µg/200 µl m 2
(0)
RAW 309 Cr.1 + LPS Cell Lysate sc-24770 500 µg/200 µl m
(1)
SK-BR-3 + PMA Cell Lysate sc-24773 500 µg/200 µl h
(0)
WiDr Cell Lysate sc-24779 500 µg/200 µl h
(0)
A-431 + EGF Whole Cell Lysate sc-2202 500 µg/200 µl h 1,716
(1)
HeLa + Calyculin A Cell Lysate sc-2271 500 µg/200 µl h
(1)
HeLa + heat shock Cell Lysate sc-2272 500 µg/200 µl h
(0)
HeLa + IFN-α Cell Lysate sc-2273 500 µg/200 µl h
(0)
HeLa + IFN-γ Cell Lysate sc-2222 500 µg/200 µl h 4
(0)
HeLa + PMA Cell Lysate sc-2258 500 µg/200 µl h
(0)
HeLa + TNFα Cell Lysate sc-2228 500 µg/200 µl h
(0)
HeLa + UV irradiated Cell Lysate sc-2221 500 µg/200 µl h 2
(1)
HeLa + nocodazole Cell Lysate sc-2274 500 µg/200 µl h
(0)
Hep G2 Cell Lysate sc-2227 500 µg/200 µl h 21
(5)
HISM Cell Lysate sc-2229 500 µg/200 µl h 3
(0)
HL-60 Whole Cell Lysate sc-2209 500 µg/200 µl h 14
(0)
HOS Cell Lysate sc-2275 500 µg/200 µl h
(0)
Hs68 Cell Lysate sc-2230 500 µg/200 µl h 2
(0)
HuT 78 Whole Cell Lysate sc-2208 500 µg/200 µl h 667
(0)
IMR-32 Cell Lysate sc-2409 500 µg/200 µl h 5
(1)
J774.A1 Cell Lysate sc-3802 500 µg/200 µl m
(0)
JAR Cell Lysate sc-2276 500 µg/200 µl h 4
(0)
Jurkat + Calyculin A Cell Lysate sc-2277 500 µg/200 µl h 1
(0)
Jurkat + IL-2 Cell Lysate sc-2278 500 µg/200 µl h
(0)
Jurkat Whole Cell Lysate sc-2204 500 µg/200 µl h 29
(1)
RAW 264.7 Whole Cell Lysate sc-2211 500 µg/200 µl m 13
(1)
HEK293T Whole Cell Lysate sc-45137 500 µg/200 µl h 1
(0)
HEK293 Whole Cell Lysate sc-45136 500 µg/200 µl h
(0)
HCT-116 Whole Cell Lysate sc-364175 500 µg/200 µl h
(0)
COLO 205 Whole Cell Lysate sc-364177 500 µg/200 µl h
(0)
A2058 Whole Cell Lysate sc-364178 500 µg/200 µl h
(0)
NCI-H292 Whole Cell Lysate sc-364179 500 µg/200 µl h
(0)
HUV-EC-C Whole Cell Lysate sc-364180 500 µg/200 µl h
(0)
NTERA-2 cl.D1 Whole Cell Lysate sc-364181 500 µg/200 µl h 1
(1)
AML-193 Whole Cell Lysate sc-364182 500 µg/200 µl h
(0)
HT-1080 Whole Cell Lysate sc-364183 500 µg/200 µl h
(0)
MDA-MB-435S Whole Cell Lysate sc-364184 500 µg/200 µl h
(0)
Neuro-2A Whole Cell Lysate sc-364185 500 µg/200 µl m
(0)
c4 Whole Cell Lysate sc-364186 500 µg/200 µl m
(0)
EOC 20 Whole Cell Lysate sc-364187 500 µg/200 µl m
(0)
C2C12 Whole Cell Lysate sc-364188 500 µg/200 µl m
(0)
LADMAC Whole Cell Lysate sc-364189 500 µg/200 µl m 1
(0)
OV-90 Whole Cell Lysate sc-364191 500 µg/200 µl h
(0)
RPMI2650 Whole Cell Lysate sc-364192 500 µg/200 µl h
(0)
TT Whole Cell Lysate sc-364195 500 µg/200 µl h
(0)
L6 Whole Cell Lysate sc-364196 500 µg/200 µl r
(0)
NRK Whole Cell Lysate sc-364197 500 µg/200 µl r
(0)

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