Ramos + IL-4 Cell Lysate: sc-24762
- 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
- human whole cell lysate; Burkitt's lymphoma cells induced with IL-4
- whole cell lysate provided as Western blotting positive control
- should be stored at -20°C and repeated freezing and thawing should be minimized
- sample vial should be placed at 95° C for up to 5 minutes, once prior to use
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SEE ALSO...
Ramos + IL-4 Cell Lysate is derived from the Ramos cell linea B-cell Burkitt's lymphoma linetreated with Interleukin-4 (IL-4). This treatment is pivotal for research as IL-4 plays a significant role in B-cell differentiation and survival. This specific lysate is rich in signaling molecules and proteins that are upregulated or modified in response to IL-4 exposure, making it a valuable tool for studying the IL-4 mediated signaling pathways. Researchers utilize this lysate to investigate the Jak/STAT pathway, which is prominently activated by IL-4. This pathway's activation leads to transcriptional changes important for B-cell proliferation and isotype switching. The lysate also aids in exploring the PI3K/Akt pathway, another crucial signaling mechanism influenced by IL-4, involved in cell survival and growth. By employing this lysate in Western blotting and immunoprecipitation experiments, scientists can examine the protein expression profiles and interactions that occur in response to IL-4, providing insights into the molecular mechanisms underlying B-cell responses to cytokines. This research tool thus facilitates a deeper understanding of the intracellular signaling events in B-cells, enhancing the knowledge of immune system regulation and lymphoma biology.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ramos + IL-4 Cell Lysate | sc-24762 | 500 µg/200 µl | $118.00 |