NIH/3T3 + PDGF Cell Lysate: sc-3803

NIH/3T3 + PDGF cell lysate is derived from NIH/3T3 cells, a mouse embryonic fibroblast cell line, treated with platelet-derived growth factor (PDGF). This lysate is extensively used in scientific research to study the mechanisms of PDGF signaling and its effects on cell proliferation, migration, and survival. PDGF binds to its receptor, PDGFR, activating downstream signaling pathways such as the PI3K/Akt, Ras-MAPK/ERK, and PLCγ pathways. Researchers utilize NIH/3T3 + PDGF cell lysate to investigate these signaling pathways, focusing on protein phosphorylation events and the regulation of transcription factors like AP-1 and STATs, which are crucial for gene expression and cellular responses. This lysate facilitates the study of PDGF-induced cellular behaviors, including cytoskeletal rearrangements and cell migration, important for understanding wound healing and tissue regeneration processes. Additionally, it is employed to assess the impact of various chemical modulators on PDGF signaling, providing insights into the regulatory mechanisms controlling cell growth and differentiation. Recent research has leveraged this lysate for proteomic and genomic analyses to identify novel signaling molecules and pathways associated with PDGF stimulation. By offering detailed insights into the molecular mechanisms of PDGF signaling, NIH/3T3 + PDGF cell lysate remains a vital resource for advancing research in cell biology and signaling pathways.