RAW 264.7 + TNFα Cell Lysate: sc-24768

RAW 264.7 + TNFα cell lysate is derived from the RAW 264.7 murine macrophage cell line treated with tumor necrosis factor-alpha (TNFα), a cytokine central to inflammatory responses. This lysate is extensively used to explore the role of TNFα in activating macrophages and its effects on inflammatory signaling pathways. Researchers use this lysate to study the activation of NF-κB and MAPK pathways, which are pivotal in mediating TNFα-induced transcription of inflammatory cytokines such as IL-6 and IL-1β. These pathways also regulate the expression of adhesion molecules and the production of reactive oxygen species (ROS), key components of the macrophage response to infection and injury. Additionally, RAW 264.7 + TNFα cell lysate allows for the examination of apoptosis and necroptosis, cell death processes that TNFα can initiate under certain conditions. This lysate aids in understanding how TNFα influences macrophage functionality and survival, providing insights into the complex network of signaling events that govern immune responses.