RKO Whole Cell Lysate: sc-364793
- 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
- human whole cell lysate; colonic carcinoma cells
- whole cell lysate provided as Western blotting positive control
- should be stored at -20°C and repeated freezing and thawing should be minimized
- sample vial should be placed at 95° C for up to 5 minutes, once prior to use
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RKO whole cell lysate is derived from the RKO cell line, a human colorectal carcinoma line valuable for studying the genetic and molecular mechanisms underpinning colorectal cancer, particularly those related to DNA mismatch repair. This lysate facilitates comprehensive studies on the cellular effects of genetic mutations common in colorectal carcinoma, such as those in the MLH1 and MSH2 genes, which play crucial roles in DNA repair processes. Researchers employ RKO lysate to explore the pathways involved in apoptosis and cell cycle arrestkey responses to DNA damage. The lysate is instrumental in examining the p53 pathway activation, commonly altered in colorectal cancers, which affects cellular outcomes like growth suppression and apoptosis. Additionally, it provides insights into the response of colorectal cancer cells to environmental stressors and chemotherapeutic agents, aiding in the understanding of cellular resilience and susceptibility. By utilizing RKO cell lysate, scientists gain detailed insights into the biochemical pathways and cellular behaviors characteristic of colorectal cancer cells in a research context, focusing purely on advancing basic scientific knowledge in cancer biology.
RKO Whole Cell Lysate References:
- RNA-binding proteins regulate the expression of the immune activating ligand MICB. | Nachmani, D., et al. 2014. Nat Commun. 5: 4186. PMID: 24924487
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RKO Whole Cell Lysate | sc-364793 | 500 µg/200 µl | $118.00 |