F9 Cell Lysate: sc-2245

F9 cell lysate is derived from the F9 teratocarcinoma cell line, widely used in developmental biology research to study the differentiation and developmental pathways of embryonic cells. This cell line is particularly valuable for examining the molecular mechanisms underlying cell differentiation towards parietal endoderm, a critical early embryonic tissue. Researchers utilize F9 cell lysate to investigate the signaling pathways, such as the retinoic acid and Wnt pathways, that regulate gene expression during differentiation. The lysate allows for the exploration of transcription factors like GATA4 and GATA6, which are essential for endodermal development. By studying protein interactions and modifications in this lysate, scientists gain insights into the epigenetic regulation of gene expression and the impact of extrinsic signals on cell fate decisions. Additionally, the F9 cell lysate serves as a model for understanding cellular responses to developmental cues, providing fundamental knowledge on cellular processes that govern early embryogenesis. This research is conducted purely within a scientific context, focusing on basic mechanisms, thereby contributing significantly to the field of developmental biology.

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F9 Cell Lysate References:

1. Down-regulation of phospholipase D during differentiation of mouse F9 teratocarcinoma cells.  |  Min, DS., et al. 1999. FEBS Lett. 454: 197-200. PMID: 10431806
2. p67 isoform of mouse disabled 2 protein acts as a transcriptional activator during the differentiation of F9 cells.  |  Cho, SY., et al. 2000. Biochem J. 352 Pt 3: 645-50. PMID: 11104669
3. JDP2, a repressor of AP-1, recruits a histone deacetylase 3 complex to inhibit the retinoic acid-induced differentiation of F9 cells.  |  Jin, C., et al. 2002. Mol Cell Biol. 22: 4815-26. PMID: 12052888
4. Teratocarcinoma F9 cells induced to differentiate with sodium butyrate produce both tissue-type and urokinase-type plasminogen activators.  |  Takeda, M., et al. 1992. J Cell Biochem. 49: 284-9. PMID: 1644865
5. Accelerated biosynthesis of neolacto-series glycosphingolipids in differentiated mouse embryonal carcinoma F9 cells detected by using dodecyl N-acetylglucosaminide as a saccharide primer.  |  Ogasawara, N., et al. 2011. J Biochem. 149: 321-30. PMID: 21148159
6. Association of human papillomavirus types 16 and 18 E6 proteins with p53.  |  Werness, BA., et al. 1990. Science. 248: 76-9. PMID: 2157286
7. Molecular cloning of the mouse cell adhesion molecule uvomorulin: cDNA contains a B1-related sequence.  |  Schuh, R., et al. 1986. Proc Natl Acad Sci U S A. 83: 1364-8. PMID: 2419906
8. Further structural studies on the F9 (T/t) antigen(s).  |  Vitetta, ES., et al. 1977. Eur J Immunol. 7: 826-9. PMID: 590322
9. A class of large polysaccharides contains the antigenic determinants for the cytotoxic antibodies in a conventional syngeneic anti-F9 serum as well as a monoclonal antibody prepared against F9 cells.  |  Iwakura, Y., et al. 1983. Cell Differ. 13: 41-8. PMID: 6194903
10. SV40 T antigen binds specifically to a cellular 53 K protein in vitro.  |  McCormick, F., et al. 1981. Nature. 292: 63-5. PMID: 6268984
11. Characterization of the F9 antigen(s) isolated from teratocarcinoma cell culture medium.  |  McCormick, PJ., et al. 1982. Cell Differ. 11: 135-40. PMID: 7116455
12. The expression of megalin (gp330) and LRP diverges during F9 cell differentiation.  |  Czekay, RP., et al. 1995. J Cell Sci. 108 (Pt 4): 1433-41. PMID: 7615664
13. L-14 lectin recognition of laminin and its promotion of in vitro cell adhesion.  |  Zhou, Q. and Cummings, RD. 1993. Arch Biochem Biophys. 300: 6-17. PMID: 8380972