BT-20 Cell Lysate: sc-2223
- 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
- human whole cell lysate; breast carcinoma cells
- whole cell lysate provided as Western blotting positive control
- should be stored at -20°C and repeated freezing and thawing should be minimized
- sample vial should be placed at 95° C for up to 5 minutes, once prior to use
QUICK LINKS
SEE ALSO...
The BT-20 cell lysate is derived from the BT-20 human breast carcinoma cell line, originally isolated from the malignant tissue of a 74-year-old female patient. This lysate encompasses a complex array of cellular materials including proteins, RNA, DNA, and various biochemicals that collectively represent the molecular composition of the cancer cells. Researchers use BT-20 lysates primarily to study the biology and biochemistry of breast cancer cells, focusing on aspects such as cell proliferation, gene expression, and protein synthesis. The BT-20 cell line, and consequently its lysate, is particularly significant for exploring mechanisms of cancer cell resistance and susceptibility to different environmental conditions or experimental treatments. In scientific studies, the lysate has been instrumental in proteomic and genomic research, providing insights into the molecular pathways that are active in breast cancer cells. By analyzing these lysates, scientists can delve into the cellular machinery and interactions at a molecular level, aiding in the understanding of how breast cancer cells operate and respond to various stimuli. This makes BT-20 cell lysate a valuable resource for non-clinical experimental research aimed at elucidating the complex biological processes involved in the pathology of breast cancer.
BT-20 Cell Lysate References:
- Exocytosis of active cathepsin B enzyme activity at pH 7.0, inhibition and molecular mass. | Linebaugh, BE., et al. 1999. Eur J Biochem. 264: 100-9. PMID: 10447678
- Analysis of p53 expression in human tumours: an antibody raised against human p53 expressed in Escherichia coli. | Midgley, CA., et al. 1992. J Cell Sci. 101 (Pt 1): 183-9. PMID: 1569122
- Human tumor cell lysates as a protein source for the detection of cancer antigen-specific humoral immunity. | Goodell, V. and Disis, ML. 2005. J Immunol Methods. 299: 129-38. PMID: 15914197
- Expression of motility-related protein MRP1/CD9, N-cadherin, E-cadherin, alpha-catenin and beta-catenin in retinoblastoma. | Mohan, A., et al. 2007. Exp Eye Res. 84: 781-9. PMID: 17316610
- Humoral immunity directed against tumor-associated antigens as potential biomarkers for the early diagnosis of cancer. | Lu, H., et al. 2008. J Proteome Res. 7: 1388-94. PMID: 18311901
- Selectin ligand sialyl-Lewis x antigen drives metastasis of hormone-dependent breast cancers. | Julien, S., et al. 2011. Cancer Res. 71: 7683-93. PMID: 22025563
- Mac-2 binding protein is a novel E-selectin ligand expressed by breast cancer cells. | Shirure, VS., et al. 2012. PLoS One. 7: e44529. PMID: 22970241
- PARP3 inhibitors ME0328 and olaparib potentiate vinorelbine sensitization in breast cancer cell lines. | Sharif-Askari, B., et al. 2018. Breast Cancer Res Treat. 172: 23-32. PMID: 30039287
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BT-20 Cell Lysate | sc-2223 | 500 µg/200 µl | $118.00 |