A-431 + Calyculin A Cell Lysate: sc-2260

The A-431 + Calyculin A cell lysate originates from the A-431 cell line, an epithelial carcinoma cell line derived from the epidermoid carcinoma of an 85-year-old female. This particular lysate is treated with Calyculin A, a potent inhibitor of protein phosphatases 1 and 2A, which are critical in the regulation of cellular processes through dephosphorylation. This treatment effectively preserves the phosphorylation state of proteins within the cells at the time of lysate preparation, making it particularly valuable in the study of signaling pathways that are modulated by phosphorylation. The A-431 cells, known for their robust expression of epidermal growth factor receptor (EGFR), offer a rich system for examining the dynamics of EGFR signaling pathways, especially when altered by pharmacological agents like Calyculin A. Researchers utilize this lysate in various non-clinical, non-therapeutic research contexts, mainly focusing on basic molecular and cellular biology studies. For instance, it facilitates the exploration of the mechanisms behind protein phosphorylation impacts on cellular behavior, aids in the identification of kinase targets, and helps in understanding the cellular response to external signaling molecules. Overall, A-431 + Calyliculin A cell lysate serves as a crucial tool in proteomics and biochemistry for dissecting the roles of phosphorylation in cellular processes, thereby enriching our understanding of cellular communication and regulation mechanisms.