HCT-8 Cell Lysate: sc-24675

HCT-8 cell lysate is derived from the HCT-8 cell line, a human ileocecal adenocarcinoma line used extensively in cancer research to study tumor biology and cellular mechanisms. This cell line is particularly valuable for exploring the processes of cell adhesion, migration, and invasion, which are critical for understanding metastasis in colorectal cancer. Researchers utilize HCT-8 lysate to investigate the molecular pathways involved in these processes, including the Wnt/β-catenin, PI3K/Akt, and MAPK/ERK pathways. These pathways are essential for regulating cell proliferation, survival, and differentiation in cancerous cells. The HCT-8 lysate is also used to study epithelial-mesenchymal transition (EMT), a key process in cancer metastasis, by examining the expression and activity of EMT markers such as E-cadherin, N-cadherin, and vimentin. Additionally, this lysate aids in the analysis of how cancer cells respond to various chemotherapeutic agents and targeted therapies, providing insights into mechanisms of drug resistance and sensitivity. Through proteomic and genomic analyses, HCT-8 whole cell lysate contributes to a deeper understanding of the molecular alterations that drive colorectal cancer progression.

HCT-8 Cell Lysate References:

1. AKT proto-oncogene overexpression is an early event during sporadic colon carcinogenesis.  |  Roy, HK., et al. 2002. Carcinogenesis. 23: 201-5. PMID: 11756242
2. Identification of heat shock protein 90α as an IMH-2 epitope-associated protein and correlation of its mRNA overexpression with colorectal cancer metastasis and poor prognosis.  |  Chen, WS., et al. 2011. Int J Colorectal Dis. 26: 1009-17. PMID: 21519806
3. TRIM6 promotes colorectal cancer cells proliferation and response to thiostrepton by TIS21/FoxM1.  |  Zheng, S., et al. 2020. J Exp Clin Cancer Res. 39: 23. PMID: 31992359