SW480 Cell Lysate: sc-2219

SW480 Cell Lysate is derived from SW480 cells, a human colorectal adenocarcinoma cell line established from the primary tumor of a 50-year-old male patient with Duke's stage B colorectal carcinoma. This lysate is extensively used in cancer research to investigate the molecular mechanisms underlying colorectal cancer progression and metastasis. SW480 cells harbor mutations in key oncogenes and tumor suppressor genes, such as KRAS and APC, making the lysate valuable for studying signaling pathways involved in tumor growth, survival, and differentiation. Researchers utilize SW480 Cell Lysate to analyze the expression, phosphorylation, and interactions of proteins involved in critical pathways such as Wnt/β-catenin, PI3K/AKT, and MAPK/ERK using techniques like western blotting, immunoprecipitation, and mass spectrometry. This lysate is particularly useful for examining the effects of these genetic alterations on cell cycle regulation, apoptosis, and epithelial-mesenchymal transition (EMT), which are essential for tumor invasion and metastasis. Additionally, SW480 Cell Lysate aids in exploring the cellular responses to various chemical compounds and growth factors, providing insights into the modulation of signaling pathways that drive colorectal cancer progression. By comparing SW480 lysates with those from normal colonic epithelial cells, scientists can identify specific molecular alterations associated with colorectal carcinoma, advancing research in cancer biology and signal transduction.

SW480 Cell Lysate References:

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