XP12RO Whole Cell Lysate: sc-364364

XP12RO Whole Cell Lysate is derived from XP12RO cells, a fibroblast cell line obtained from a patient with Xeroderma Pigmentosum (XP), specifically from complementation group A. This lysate is extensively used in research to study the molecular mechanisms of DNA repair, particularly the nucleotide excision repair (NER) pathway. XP12RO cells lack functional XP-A protein, which is crucial for recognizing and repairing UV-induced DNA damage. Researchers utilize XP12RO Whole Cell Lysate to investigate the effects of UV radiation on cellular components and to analyze the impaired NER pathway using techniques like western blotting, immunoprecipitation, and proteomics. The lysate helps in identifying protein interactions and post-translational modifications involved in DNA damage response. Additionally, it is employed in studies aimed at understanding the compensatory mechanisms that cells may adopt in the absence of functional NER. By comparing XP12RO lysates with those from normal fibroblasts, scientists gain insights into the cellular responses to DNA damage and the role of XP-A in maintaining genomic integrity. This lysate also aids in screening chemical compounds that could influence DNA repair pathways, making it a valuable tool for advancing our understanding of genetic stability and mutagenesis.