COS Whole Cell Lysate: sc-364228

The COS Whole Cell Lysate is derived from COS cells, a fibroblast-like cell line that originates from African green monkey kidney tissue. This cell line was engineered to contain the SV40 virus large T antigen, which helps to drive the replication of plasmids containing the SV40 origin of replication, making it particularly useful for various molecular biology applications. The lysate itself includes a comprehensive mixture of cellular components such as proteins, nucleic acids, lipids, and metabolites, obtained through methods like sonication, detergent lysis, or enzymatic digestion. In research, the COS Whole Cell Lysate is extensively utilized to study protein expression and post-translational modifications, providing insights into cellular processes such as protein trafficking, signal transduction, and gene regulation. This lysate is especially valuable for transient transfection studies, where researchers introduce foreign DNA into the cells and analyze protein interactions and functions within a non-human system. Additionally, the unique properties of COS cells, including their high protein synthesis capacity and rapid growth rate, make the derived lysate an excellent tool for biochemical assays and protein production, aiding in the elucidation of complex biological mechanisms in a purely research-focused context.