TE671 Cell Lysate: sc-2416
- 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
- human whole cell lysate; rhabdomyosarcoma cells
- whole cell lysate provided as Western blotting positive control
- should be stored at -20°C and repeated freezing and thawing should be minimized
- sample vial should be placed at 95° C for up to 5 minutes, once prior to use
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TE671 Cell Lysate is derived from TE671 cells, a human rhabdomyosarcoma cell line originally established from the muscle tissue of a 7-year-old female patient. This lysate is widely used in cancer research to investigate the molecular mechanisms underlying rhabdomyosarcoma, a type of soft tissue sarcoma. TE671 cells exhibit characteristic genetic and epigenetic alterations, including changes in oncogenes such as MYOD1 and PAX3-FOXO1 fusion genes, making the lysate valuable for studying signaling pathways involved in tumor growth, differentiation, and metastasis. Researchers utilize TE671 Cell Lysate to analyze the expression, phosphorylation, and interactions of key proteins involved in pathways such as PI3K/AKT, MAPK/ERK, and mTOR using techniques like western blotting, immunoprecipitation, and mass spectrometry. This lysate is particularly useful for examining the molecular basis of muscle cell differentiation by analyzing the roles of muscle-specific transcription factors and signaling molecules. Additionally, it aids in exploring the cellular responses to various chemical compounds and growth factors that influence cell proliferation, apoptosis, and differentiation. By comparing TE671 lysates with those from normal muscle cells, scientists can identify specific molecular alterations associated with rhabdomyosarcoma, advancing research in muscle biology and cancer signal transduction.
TE671 Cell Lysate References:
- One step generation of fully chimeric antibodies using Cgamma1- and Ckappa mutant mice. | Jensen, M., et al. 2007. J Immunother. 30: 338-49. PMID: 17414325
- Ordered assembly of murine leukemia virus capsid protein on lipid nanotubes directs specific binding by the restriction factor, Fv1. | Hilditch, L., et al. 2011. Proc Natl Acad Sci U S A. 108: 5771-6. PMID: 21436027
- K-Ras and cyclooxygenase-2 coactivation augments intraductal papillary mucinous neoplasm and Notch1 mimicking human pancreas lesions. | Chiblak, S., et al. 2016. Sci Rep. 6: 29455. PMID: 27381829
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TE671 Cell Lysate | sc-2416 | 500 µg/200 µl | $118.00 |