U-937 + TNFα Cell Lysate: sc-2297

U-937 + TNFα Cell Lysate is derived from U-937 cells, a human histiocytic lymphoma cell line, treated with Tumor Necrosis Factor-alpha (TNFα). This lysate is utilized to study the molecular mechanisms of inflammation, apoptosis, and signal transduction in cancer research. TNFα is a cytokine that activates the NF-κB pathway, leading to the transcription of genes involved in immune and inflammatory responses. Researchers use U-937 + TNFα Cell Lysate to analyze the changes in protein expression, phosphorylation, and interaction following TNFα stimulation. Techniques such as western blotting, immunoprecipitation, and mass spectrometry are employed to study key signaling molecules including IκB, RelA/p65, and various MAPKs. This lysate is particularly useful for investigating the dynamics of NF-κB activation and the subsequent cellular responses, such as apoptosis and cytokine production. Additionally, it aids in exploring the crosstalk between TNFα-induced pathways and other signaling cascades like JAK/STAT and PI3K/AKT. By comparing this lysate with untreated U-937 cell lysates, scientists can identify specific molecular alterations induced by TNFα, advancing the understanding of inflammatory processes and their role in lymphoma progression. This research is crucial for uncovering potential molecular targets for further study in cancer biology.