NTERA-2 cl.D1 Whole Cell Lysate: sc-364181

NTERA-2 cl.D1 whole cell lysate is derived from the NTERA-2 cl.D1 cell line, a pluripotent human embryonal carcinoma cell line originally isolated from a testicular carcinoma. This lysate is extensively used in research to study neuronal differentiation, pluripotency, and cellular signaling pathways. NTERA-2 cl.D1 cells are known for their ability to differentiate into neuron-like cells upon treatment with retinoic acid, making the lysate an invaluable tool for investigating mechanisms of neurogenesis and stem cell biology. Researchers utilize NTERA-2 cl.D1 whole cell lysate to analyze the activation and regulation of key signaling pathways, including the Wnt/β-catenin, Notch, and BMP pathways, which are crucial for cell fate determination and differentiation. This lysate is also employed to study the expression and post-translational modifications of pluripotency markers such as OCT4, SOX2, and NANOG, as well as neural-specific markers like MAP2 and TUJ1. Additionally, it is used to evaluate the effects of various chemical modulators on these pathways, providing insights into the molecular mechanisms governing stem cell maintenance and differentiation. Recent research has leveraged this lysate for proteomic and transcriptomic analyses to identify novel regulators and signaling networks involved in pluripotency and differentiation. NTERA-2 cl.D1 whole cell lysate thus remains a critical resource for advancing stem cell and neurobiological research.