NTERA-2 cl.D1 Whole Cell Lysate whole cell lysate provided as Western blotting positive control

NTERA-2 cl.D1 Whole Cell Lysate: sc-364181

NTERA-2 cl.D1 Whole Cell Lysate is rated 1.0 out of 5 by 1.
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Datasheets

    • 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
    • human whole cell lysate; embryonal carcinoma cells
    • whole cell lysate provided as Western blotting positive control
    • should be stored at -20°C and repeated freezing and thawing should be minimized
    • sample vial should be boiled once prior to use
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Rated 1 out of 5 by from little/no protein in lysate Shipment arrived completely thawed on blue ice. No sign of protein in NTera-2 lysate by Ponceau stain, and no bands for tubulin in NTera-2 sample (which was readily detectable in equal mass of protein from HEK293 cell lysate (sc-45136) on same blot). Unable to detect Oct4 in this lysate either. Batch either contained no protein, or had degraded entirely by time it was shipped.
Date published: 2017-02-22
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