HL-60 Whole Cell Lysate: sc-2209

The HL-60 Whole Cell Lysate is derived from the HL-60 cell line, a widely utilized resource in biochemical and genetic research, especially valuable in the study of hematopoietic and immune system processes. Originating from a human promyelocytic leukemia patient in the 1970s, this cell line has since become a model for studying myeloid differentiation and cellular metabolism. The lysate itself is prepared by disrupting HL-60 cells to release their intracellular components, which include proteins, nucleic acids, and small metabolites. This complex mixture is a snapshot of the cellular environment and is extensively used in proteomics and genomics to analyze cell signaling pathways and gene expression profiles. In research settings, HL-60 whole cell lysates serve as a crucial comparative tool for understanding cellular responses under various experimental conditions, facilitating insights into molecular dynamics and cellular responses without direct implications for human application. The versatility of this lysate makes it an indispensable component in laboratories focusing on basic science research, helping to explain fundamental biological processes and cellular mechanisms that underpin more complex biological systems and behaviors. Through such studies, researchers can explore the complex web of cellular activities that define human cell biology in a controlled, observable environment.

HL-60 Whole Cell Lysate References:

1. Motility-related proteins as markers for head and neck squamous cell cancer.  |  Abraham, MT., et al. 2001. Laryngoscope. 111: 1285-9. PMID: 11568556
2. Biomolecular characterization of CD44-fibrin(ogen) binding: distinct molecular requirements mediate binding of standard and variant isoforms of CD44 to immobilized fibrin(ogen).  |  Alves, CS., et al. 2009. J Biol Chem. 284: 1177-89. PMID: 19004834
3. Distinct kinetic and molecular requirements govern CD44 binding to hyaluronan versus fibrin(ogen).  |  Raman, PS., et al. 2012. Biophys J. 103: 415-423. PMID: 22947857
4. PDGF suppresses the sulfation of CD44v and potentiates CD44v-mediated binding of colon carcinoma cells to fibrin under flow.  |  Alves, CS. and Konstantopoulos, K. 2012. PLoS One. 7: e41472. PMID: 23056168
5. Proteome-wide discovery and characterizations of nucleotide-binding proteins with affinity-labeled chemical probes.  |  Xiao, Y., et al. 2013. Anal Chem. 85: 3198-206. PMID: 23413923
6. The RNA/DNA-binding protein PSF relocates to cell membrane and contributes cells′ sensitivity to antitumor drug, doxorubicin.  |  Ren, S., et al. 2014. Cytometry A. 85: 231-41. PMID: 24327337
7. Molecular analysis of the mouse brain exposed to chronic mild stress: The influence of hepatocyte nuclear factor 4α on physiological homeostasis.  |  Ikubo, K., et al. 2017. Mol Med Rep. 16: 301-309. PMID: 28498421
8. Regulation of GATA-2 phosphorylation by mitogen-activated protein kinase and interleukin-3.  |  Towatari, M., et al. 1995. J Biol Chem. 270: 4101-7. PMID: 7876160