A-431 + AP Cell Lysate: sc-24651
- 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
- human whole cell lysate; epidermoid carcinoma cells treated with alkaline phosphatase (AP)
- whole cell lysate provided as Western blotting positive control
- should be stored at -20°C and repeated freezing and thawing should be minimized
- sample vial should be placed at 95° C for up to 5 minutes, once prior to use
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The A-431 + AP cell lysate is derived from the A-431 cell line, which is a well-known epithelial carcinoma cell line originally established from the epidermoid carcinoma of a 85-year-old female patient. This particular lysate is unique as it includes an additional modification with alkaline phosphatase (AP), an enzyme that dephosphorylates proteins, which can be pivotal in studies involving signaling pathways. The A-431 cells are characterized by high levels of epidermal growth factor receptor (EGFR), making them a valuable model for research in signal transduction and cancer biology. The combination of A-431 cells with AP in the lysate allows researchers to investigate the effects of dephosphorylation on cell signaling mechanisms and protein interactions. This lysate is extensively utilized in basic research, particularly to understand the dynamics of protein phosphorylation and its implications in cellular processes such as proliferation and differentiation. By providing a complex mixture of cellular components, the A-431 + AP cell lysate serves as a critical tool in proteomics for identifying protein modifications and interactions, and in biochemical assays where understanding the impact of phosphorylation on protein function is essential. The lysate thus supports a broad range of research applications that are fundamental in elucidating cellular behavior and protein functionality.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
A-431 + AP Cell Lysate | sc-24651 | 500 µg/200 µl | $118.00 |