A-10 Cell Lysate: sc-3806

The A-10 Cell Lysate is a valuable research reagent derived from the A-10 cell line, which originates from rat thoracic aorta and is extensively used to study vascular smooth muscle cells. This lysate is prepared through a detailed process of culturing A-10 cells under optimal conditions, followed by lysis to release intracellular proteins, nucleic acids, and other cellular components. Researchers utilize A-10 Cell Lysate to investigate a wide range of cellular processes, including signal transduction, gene expression, and cytoskeletal dynamics, which are crucial in understanding vascular physiology and pathology. The lysate is particularly useful in studies involving the mechanisms of smooth muscle contraction, cellular responses to vasoactive agents, and the regulation of blood pressure. Techniques such as Western blotting, proteomics, and transcriptomics frequently employ A-10 Cell Lysate to analyze protein interactions, post-translational modifications, and gene expression profiles. This lysate also aids in validating antibodies and other experimental reagents, ensuring the accuracy and reproducibility of scientific findings. By providing a rich source of cellular material, A-10 Cell Lysate significantly contributes to advancing knowledge in vascular biology, helping to explain the complex molecular and cellular mechanisms that govern smooth muscle cell function and vascular health.

A-10 Cell Lysate References:

1. A novel homocysteine-responsive gene, smap8, modulates mitogenesis in rat vascular smooth muscle cells.  |  Nishimoto, S., et al. 2003. Eur J Biochem. 270: 2521-31. PMID: 12755708
2. Isolation and characterization of primary canine lens epithelial cells.  |  Long, AC., et al. 2008. Vet Ophthalmol. 11: 38-42. PMID: 18190351
3. Tenascin-C enhances crosstalk signaling of integrin αvβ3/PDGFR-β complex by SRC recruitment promoting PDGF-induced proliferation and migration in smooth muscle cells.  |  Ishigaki, T., et al. 2011. J Cell Physiol. 226: 2617-24. PMID: 21792920