Caco-2 Cell Lysate: sc-2262
- 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
- human whole cell lysate; adenocarcinoma, colorectal cells
- whole cell lysate provided as Western blotting positive control
- should be stored at -20°C and repeated freezing and thawing should be minimized
- sample vial should be placed at 95° C for up to 5 minutes, once prior to use
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The Caco-2 Cell Lysate is prepared from the Caco-2 cell line, originally derived from human colorectal carcinoma cells. This lysate contains a rich mixture of cellular components, including proteins, nucleic acids, and other molecular entities, extracted through methods such as chemical lysis, physical disruption, or enzymatic digestion. The Caco-2 cell line is epithelial-like and is extensively used as a model to study the enteric properties of epithelial cells in the intestine. In research, the Caco-2 Cell Lysate has proven invaluable for studying cellular processes such as absorption, transport, and barrier function of intestinal cells. It facilitates a wide range of studies focusing on cellular metabolism, protein-protein interactions, and the cellular response to dietary components or environmental stressors. Additionally, it serves as a critical reference point in proteomics for understanding protein expression patterns and identifying biomolecular interactions specific to epithelial cells. By providing a snapshot of the cellular environment of intestinal epithelial cells, Caco-2 Cell Lysate supports fundamental research in cell biology, helping to explain mechanisms of cellular function and interaction in a non-clinical context. This lysate thus significantly contributes to the broader understanding of epithelial cell biology and the complex interplay of cellular processes within these cells.
Caco-2 Cell Lysate References:
- Metabolism of flavonoids via enteric recycling: mechanistic studies of disposition of apigenin in the Caco-2 cell culture model. | Hu, M., et al. 2003. J Pharmacol Exp Ther. 307: 314-21. PMID: 12893842
- Disposition mechanisms of raloxifene in the human intestinal Caco-2 model. | Jeong, EJ., et al. 2004. J Pharmacol Exp Ther. 310: 376-85. PMID: 15020665
- Mechanistic studies on protopanaxadiol, Rh2, and ginseng (Panax quinquefolius) extract induced cytotoxicity in intestinal Caco-2 cells. | Popovich, DG. and Kitts, DD. 2004. J Biochem Mol Toxicol. 18: 143-9. PMID: 15252870
- Absorption and metabolism of genistein and its five isoflavone analogs in the human intestinal Caco-2 model. | Chen, J., et al. 2005. Cancer Chemother Pharmacol. 55: 159-69. PMID: 15455178
- Expression, localization, and functional characteristics of breast cancer resistance protein in Caco-2 cells. | Xia, CQ., et al. 2005. Drug Metab Dispos. 33: 637-43. PMID: 15716365
- Species- and disposition model-dependent metabolism of raloxifene in gut and liver: role of UGT1A10. | Jeong, EJ., et al. 2005. Drug Metab Dispos. 33: 785-94. PMID: 15769887
- Accumulation and oriented transport of ampicillin in Caco-2 cells from its pivaloyloxymethylester prodrug, pivampicillin. | Chanteux, H., et al. 2005. Antimicrob Agents Chemother. 49: 1279-88. PMID: 15793098
- Paraoxonases (PONs) 1, 2, and 3 are expressed in human and mouse gastrointestinal tract and in Caco-2 cell line: selective secretion of PON1 and PON2. | Shamir, R., et al. 2005. Free Radic Biol Med. 39: 336-44. PMID: 15993332
- Disposition of formononetin via enteric recycling: metabolism and excretion in mouse intestinal perfusion and Caco-2 cell models. | Jeong, EJ., et al. 2005. Mol Pharm. 2: 319-28. PMID: 16053335
- Disposition of flavonoids via enteric recycling: determination of the UDP-glucuronosyltransferase isoforms responsible for the metabolism of flavonoids in intact Caco-2 TC7 cells using siRNA. | Liu, X., et al. 2007. Mol Pharm. 4: 873-82. PMID: 17927138
- A catenary model to study transport and conjugation of baicalein, a bioactive flavonoid, in the Caco-2 cell monolayer: demonstration of substrate inhibition. | Sun, H., et al. 2008. J Pharmacol Exp Ther. 326: 117-26. PMID: 18385448
- SULT1A3-mediated regiospecific 7-O-sulfation of flavonoids in Caco-2 cells can be explained by the relevant molecular docking studies. | Meng, S., et al. 2012. Mol Pharm. 9: 862-73. PMID: 22352375
- Intracellular retention and subsequent release of bovine milk lactoferrin taken up by human enterocyte-like cell lines, Caco-2, C2BBe1 and HT-29. | Akiyama, Y., et al. 2013. Biosci Biotechnol Biochem. 77: 1023-9. PMID: 23649267
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Caco-2 Cell Lysate | sc-2262 | 500 µg/200 µl | $118.00 |