C32 Whole Cell Lysate: sc-2205
- 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
- human whole cell lysate; amelanotic melanoma cells
- whole cell lysate provided as Western blotting positive control
- should be stored at -20°C and repeated freezing and thawing should be minimized
- sample vial should be placed at 95° C for up to 5 minutes, once prior to use
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The C32 Whole Cell Lysate is derived from the C32 cell line, which is a human skin-derived melanoma cell line used predominantly in research related to skin cell biology and pathology. This specific lysate is a rich mixture of cellular contents including proteins, DNA, RNA, and other cellular molecules, obtained through the thorough lysis of C32 cells. Typically, the lysis process involves physical, chemical, or enzymatic methods to break down the cell membrane and release the intracellular components. Researchers utilize the C32 Whole Cell Lysate as a crucial tool in molecular biology and biochemistry for a variety of purposes including the study of melanoma cell behaviors, protein expression, and gene regulation. It serves as an essential baseline or control in experiments aimed at understanding the molecular pathways involved in skin cell metabolism and the cellular response to different environmental conditions. Furthermore, the lysate is instrumental in assay development and the calibration of analytical techniques, helping scientists to develop more refined approaches for studying complex biochemical networks. The C32 Whole Cell Lysate thus plays a vital role in advancing our understanding of cellular processes in certain contexts, particularly those related to the biology of skin cells.
C32 Whole Cell Lysate References:
- Human RAD9 checkpoint control/proapoptotic protein can activate transcription of p21. | Yin, Y., et al. 2004. Proc Natl Acad Sci U S A. 101: 8864-9. PMID: 15184659
- Polyamines are implicated in the emergence of the embryo from obligate diapause. | Lefèvre, PL., et al. 2011. Endocrinology. 152: 1627-39. PMID: 21303959
- The thyroid hormone triiodothyronine controls macrophage maturation and functions: protective role during inflammation. | Perrotta, C., et al. 2014. Am J Pathol. 184: 230-47. PMID: 24215914
- A systematic approach to quantitative Western blot analysis. | Pillai-Kastoori, L., et al. 2020. Anal Biochem. 593: 113608. PMID: 32007473
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C32 Whole Cell Lysate | sc-2205 | 500 µg/200 µl | $118.00 |