MCF7 + EGF + β-estradiol Cell Lysate: sc-24730

MCF7 + EGF + β-estradiol cell lysate is derived from MCF7 cells, a human breast cancer cell line, treated with epidermal growth factor (EGF) and β-estradiol to study the synergistic effects of these growth and hormonal signals on cellular functions. This particular lysate provides a valuable tool for dissecting the complex interplay between growth factor signaling and estrogen receptor activation. Researchers use this lysate to examine how EGF and β-estradiol together influence the ERK and PI3K/Akt signaling pathways, which are critical for cell proliferation and survival. The combined treatment promotes a more robust activation of these pathways compared to the individual effects of EGF or β-estradiol, leading to enhanced cell cycle progression and anti-apoptotic activity. This lysate aids in studying the modulation of gene expression related to cell cycle regulators and apoptosis inhibitors, providing insights into the mechanisms of hormone-responsive and growth factor-responsive gene regulation. Furthermore, it facilitates the exploration of cross-talk between membrane-bound and nuclear hormone receptors, deepening our understanding of cellular responsiveness to combined hormonal and growth factor stimuli. By focusing on these biochemical interactions within a purely research context, the lysate contributes to our fundamental knowledge of cellular signaling dynamics in cancer biology.