HEK293T Whole Cell Lysate: sc-45137

HEK293T whole cell lysate is derived from the HEK293T cell line, a derivative of the HEK293 line that has been transfected with the SV40 large T-antigen. This modification enhances the cell's ability to support the replication of vectors carrying the SV40 origin of replication, making it a preferred model for gene expression studies. HEK293T cells are widely used in research due to their high transfectability and robust growth, which facilitate the production of large quantities of recombinant proteins. Researchers utilize HEK293T lysate to study various cellular and molecular mechanisms, including signal transduction, protein-protein interactions, and gene expression regulation. This lysate is particularly valuable for investigating the effects of genetic modifications and the function of overexpressed proteins. Key signaling pathways such as MAPK/ERK, PI3K/Akt, and Wnt/β-catenin are often analyzed using this lysate to understand their roles in cell proliferation, differentiation, and survival. HEK293T lysate is also employed in high-throughput screening assays to identify potential drug targets and to evaluate the cellular effects of novel compounds. By providing a rich source of cellular proteins, this lysate aids in proteomic studies that explore post-translational modifications and protein stability.

HEK293T Whole Cell Lysate References:

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2. Distinct activities of the alpha-catenin family, alpha-catulin and alpha-catenin, on beta-catenin-mediated signaling.  |  Merdek, KD., et al. 2004. Mol Cell Biol. 24: 2410-22. PMID: 14993280
3. Signal transducers and activators of transcription-3 binding to the fibroblast growth factor receptor is activated by receptor amplification.  |  Dudka, AA., et al. 2010. Cancer Res. 70: 3391-401. PMID: 20388777
4. The RNA helicase RHAU (DHX36) unwinds a G4-quadruplex in human telomerase RNA and promotes the formation of the P1 helix template boundary.  |  Booy, EP., et al. 2012. Nucleic Acids Res. 40: 4110-24. PMID: 22238380
5. Beyond secondary structure: primary-sequence determinants license pri-miRNA hairpins for processing.  |  Auyeung, VC., et al. 2013. Cell. 152: 844-58. PMID: 23415231
6. Structure of active dimeric human telomerase.  |  Sauerwald, A., et al. 2013. Nat Struct Mol Biol. 20: 454-60. PMID: 23474713
7. Four jointed box 1 promotes angiogenesis and is associated with poor patient survival in colorectal carcinoma.  |  Al-Greene, NT., et al. 2013. PLoS One. 8: e69660. PMID: 23922772
8. A novel mutation in GATA6 causes pancreatic agenesis.  |  Stanescu, DE., et al. 2015. Pediatr Diabetes. 16: 67-70. PMID: 24433315
9. Methyltransferase-like protein 16 binds the 3′-terminal triple helix of MALAT1 long noncoding RNA.  |  Brown, JA., et al. 2016. Proc Natl Acad Sci U S A. 113: 14013-14018. PMID: 27872311
10. CHMP1B is a target of USP8/UBPY regulated by ubiquitin during endocytosis.  |  Crespo-Yàñez, X., et al. 2018. PLoS Genet. 14: e1007456. PMID: 29933386
11. Human GTPBP10 is required for mitoribosome maturation.  |  Maiti, P., et al. 2018. Nucleic Acids Res. 46: 11423-11437. PMID: 30321378
12. The Length of a Ubiquitin Chain: A General Factor for Selective Recognition by Ubiquitin-Binding Proteins.  |  Lutz, J., et al. 2020. Angew Chem Int Ed Engl. 59: 12371-12375. PMID: 32301549
13. Human GTPBP5 (MTG2) fuels mitoribosome large subunit maturation by facilitating 16S rRNA methylation.  |  Maiti, P., et al. 2020. Nucleic Acids Res. 48: 7924-7943. PMID: 32652011
14. The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription.  |  Castañeda, AF., et al. 2020. PLoS Pathog. 16: e1008843. PMID: 32886723
15. DNA Polymerase ι Interacts with Both the TRAF-like and UBL1-2 Domains of USP7.  |  Ashton, NW., et al. 2021. J Mol Biol. 433: 166733. PMID: 33279577