CHO-K1 Cell Lysate: sc-3809

The CHO-K1 Cell Lysate is derived from the CHO-K1 cell line, a type of Chinese hamster ovary cell that is extensively used in scientific research due to its robust growth and genetic stability. This lysate contains a complex mixture of cellular components such as proteins, nucleic acids, lipids, and metabolites, extracted through various disruption methods like sonication, detergent lysis, or enzymatic cleavage. Originating from an epithelial-like cell line adapted from the ovary of the Chinese hamster, CHO-K1 cells are particularly valuable for studies involving cellular biology and genetics. The CHO-K1 Cell Lysate provides researchers with a rich resource for understanding basic cell functions, studying gene expression, and analyzing protein interactions and signaling pathways. It is instrumental in the field of biotechnology for gene expression studies, particularly because these cells are commonly used to produce recombinant proteins. Moreover, the lysate from CHO-K1 cells allows for the exploration of cellular responses to environmental stresses, aiding in the elucidation of cellular adaptation mechanisms. By providing a detailed profile of the proteins and other molecules present in the cells, CHO-K1 Cell Lysate significantly contributes to research aimed at understanding the complex molecular machinery of eukaryotic cells and their complex biological processes.

CHO-K1 Cell Lysate References:

1. Arfophilin is a common target of both class II and class III ADP-ribosylation factors.  |  Shin, OH., et al. 2001. Biochemistry. 40: 10846-52. PMID: 11535061
2. Selection and characterization of an internalizing epidermal-growth-factor-receptor antibody.  |  Zhao, X., et al. 2007. Biotechnol Appl Biochem. 46: 27-33. PMID: 16886908
3. Ligand-dependent localization and intracellular stability of sigma-1 receptors in CHO-K1 cells.  |  Mavlyutov, TA. and Ruoho, AE. 2007. J Mol Signal. 2: 8. PMID: 17883859
4. Generation and characterization of recombinant feline beta-galactosidase for preclinical enzyme replacement therapy studies in GM1 gangliosidosis.  |  Samoylova, TI., et al. 2008. Metab Brain Dis. 23: 161-73. PMID: 18421424
5. [Haponin (eIF1AD) interacts with glyceraldehyde 3-phosphate dehydrogenase in the CHO-K1 cell line].  |  Rakitina, TV., et al. 2010. Bioorg Khim. 36: 312-8. PMID: 20644585
6. Sulfation of glucuronic acid in the linkage tetrasaccharide by HNK-1 sulfotransferase is an inhibitory signal for the expression of a chondroitin sulfate chain on thrombomodulin.  |  Nakagawa, N., et al. 2011. Biochem Biophys Res Commun. 415: 109-13. PMID: 22020094
7. Monitoring the subcellular localization of doxorubicin in CHO-K1 using MEKC-LIF: liposomal carrier for enhanced drug delivery.  |  Ho, JA., et al. 2012. Talanta. 99: 683-8. PMID: 22967611
8. 5α-reductase type 3 enzyme in benign and malignant prostate.  |  Titus, MA., et al. 2014. Prostate. 74: 235-49. PMID: 24150795
9. Boronic Acid for the Traceless Delivery of Proteins into Cells.  |  Andersen, KA., et al. 2016. ACS Chem Biol. 11: 319-23. PMID: 26629587
10. Modulation of the Plasma Kallikrein-Kinin System Proteins Performed by Heparan Sulfate Proteoglycans.  |  Motta, G. and Tersariol, ILS. 2017. Front Physiol. 8: 481. PMID: 28744223
11. Amino Acid Mixture Acts as a Potent VEGF Lowering Agent in CHO-K1 Cells Exposed to High Glucose.  |  Selvi, R., et al. 2017. Arch Med Res. 48: 238-246. PMID: 28923325
12. Comprehensive Glycoproteomic Analysis of Chinese Hamster Ovary Cells.  |  Yang, G., et al. 2018. Anal Chem. 90: 14294-14302. PMID: 30457839
13. A cytoplasmic AAA family peroxin, Pex1p, interacts with Pex6p.  |  Tamura, S., et al. 1998. Biochem Biophys Res Commun. 245: 883-6. PMID: 9588209