HEK293 Whole Cell Lysate: sc-45136

HEK293 whole cell lysate is derived from the HEK293 cell line, originally generated from human embryonic kidney cells. This cell line is extensively used in biological and biomedical research due to its high transfectability and robust growth characteristics. Researchers utilize HEK293 lysate to study various cellular and molecular mechanisms, including signal transduction, gene expression, and protein-protein interactions. The cell line is particularly valuable for investigating the function of recombinant proteins, as it supports high levels of protein expression. HEK293 cells are often used to examine the activation and regulation of key signaling pathways such as MAPK/ERK, PI3K/Akt, and NF-κB, which play crucial roles in cell proliferation, survival, and apoptosis. The lysate is employed in proteomic studies to analyze protein expression patterns and post-translational modifications, providing insights into cellular responses to different stimuli and treatments. Additionally, HEK293 lysate is used to investigate the effects of genetic mutations and to screen potential drug targets in a controlled research environment. This lysate aids in understanding fundamental cellular processes and mechanisms, contributing significantly to various fields including genetics, cell biology, and pharmacology.

HEK293 Whole Cell Lysate References:

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6. Human immunodeficiency virus protein Tat induces oligodendrocyte injury by enhancing outward K+ current conducted by KV1.3.  |  Liu, H., et al. 2017. Neurobiol Dis. 97: 1-10. PMID: 27816768
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12. A weak COPI binding motif in the cytoplasmic tail of SARS-CoV-2 spike glycoprotein is necessary for its cleavage, glycosylation, and localization.  |  Jennings, BC., et al. 2021. FEBS Lett. 595: 1758-1767. PMID: 33991349
13. Expression and affinity purification of recombinant mammalian mitochondrial ribosomal small subunit (MRPS) proteins and protein-protein interaction analysis indicate putative role in tumourigenic cellular processes.  |  Revathi Paramasivam, O., et al. 2021. J Biochem. 169: 675-692. PMID: 34492114
14. Mitochondrial ribosomal small subunit (MRPS) MRPS23 protein-protein interaction reveals phosphorylation by CDK11-p58 affecting cell proliferation and knockdown of MRPS23 sensitizes breast cancer cells to CDK1 inhibitors.  |  Oviya, RP., et al. 2022. Mol Biol Rep. 49: 9521-9534. PMID: 35962848
15. O-GlcNAcylation suppresses TRAP1 activity and promotes mitochondrial respiration.  |  Kim, S., et al. 2022. Cell Stress Chaperones. 27: 573-585. PMID: 35976490