Jurkat + camptothecin Cell Lysate: sc-24713

Jurkat + Camptothecin Cell Lysate is derived from Jurkat cells, which are a type of human T lymphocyte cell line commonly used to study immune cell function and signaling. In this specific lysate, the Jurkat cells have been treated with camptothecin, a compound known for its ability to induce DNA damage by inhibiting the DNA enzyme topoisomerase I. The treatment with camptothecin triggers a cascade of cellular responses, particularly those related to DNA repair mechanisms and cell cycle regulation, making this lysate a valuable tool for studying these processes. When these cells are lysed, their internal contents are released, providing a complex mixture of proteins, nucleic acids, and other cellular components. This lysate is particularly useful for researchers who are interested in examining the pathways activated during cellular stress and the apoptotic processes consequent to DNA damage. Scientists use this lysate in various applications like Western blotting to analyze protein expression and modifications, or in kinase assays to study changes in enzyme activity. The Jurkat + Camptothecin Cell Lysate offers insights into the molecular underpinnings of cell survival and adaptation under genotoxic stress, aiding in the broader understanding of cellular response mechanisms to DNA integrity threats.