RAW 309 Cr.1 + LPS Cell Lysate: sc-24770

RAW 309 Cr.1 + LPS cell lysate is derived from the RAW 309 Cr.1 cell line, a murine macrophage line, following stimulation with lipopolysaccharide (LPS). This lysate is crucial for examining macrophage activation and the inflammatory pathways activated by LPS, a primary component of the outer membrane of Gram-negative bacteria. LPS binding to toll-like receptor 4 (TLR4) on macrophages triggers a cascade of intracellular signaling events, notably through the NF-κB and MAPK pathways, leading to the transcription and release of pro-inflammatory cytokines such as TNF-α, IL-6, and IL-1β. Researchers use RAW 309 Cr.1 + LPS cell lysate to study these pathways in detail, investigating how macrophages mediate innate immune responses and identifying key regulators within these signaling pathways. The lysate also serves to explore the dynamics of cytokine production, cellular stress responses, and the development of tolerance to repeated endotoxin exposure. By utilizing this lysate, scientists can dissect the complex interactions within macrophage signaling networks, providing a deeper understanding of immune regulation, thereby advancing basic immunological research.