NIH/3T3 + forskolin Cell Lysate: sc-24741

NIH/3T3 + forskolin cell lysate is derived from NIH/3T3 cells, a mouse embryonic fibroblast cell line, treated with forskolin. Forskolin is a diterpene that activates adenylyl cyclase, leading to an increase in cyclic AMP (cAMP) levels within the cell. This lysate is extensively used in research to study the cAMP signaling pathway and its downstream effects on cellular functions. The rise in cAMP activates protein kinase A (PKA), which then phosphorylates various target proteins, thereby regulating gene expression, cell growth, and differentiation. Researchers utilize NIH/3T3 + forskolin cell lysate to investigate the activation of PKA and its subsequent effects on signaling pathways such as the CREB (cAMP response element-binding protein) pathway. This lysate facilitates the study of phosphorylation events and the regulation of transcription factors involved in cAMP-mediated gene expression. Additionally, it is employed to examine the cross-talk between cAMP signaling and other pathways like the MAPK/ERK and PI3K/Akt pathways. Recent studies have leveraged this lysate for proteomic analyses to identify novel PKA substrates and to explore the broader network of signaling interactions influenced by cAMP. NIH/3T3 + forskolin cell lysate remains a valuable resource for advancing research in cell signaling and understanding the molecular mechanisms regulated by cAMP.