Quiescent NIH/3T3 Cell Lysate: sc-24752

Quiescent NIH/3T3 Cell Lysate is sourced from the NIH/3T3 cell line, a fibroblast cell line derived from mouse embryonic tissue, which has been rendered quiescent through serum starvation or contact inhibition. This lysate is critical in studying cellular processes under conditions of low mitogenic stimulation, which mimics the state of cells in non-proliferative phases in tissues. Researchers use this lysate to analyze cell cycle regulation and signal transduction pathways that maintain cellular quiescence. It provides valuable insights into the mechanisms of cell cycle arrest, focusing on the roles of cyclin-dependent kinase inhibitors and their regulation through various signaling pathways such as p53/p21 and TGF-β. This lysate also aids in the exploration of the cellular response to stress and the stability of the extracellular matrix, as NIH/3T3 cells are instrumental in studying fibroblast function and behavior. Additionally, the quiescent state of these cells allows for the examination of metabolic changes that occur when cells exit the cell cycle, which is crucial for understanding cell survival and adaptation in response to nutrient deprivation.