A-431 + pervanadate Cell Lysate: sc-24654
- 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
- human whole cell lysate; epidermoid carcinoma cells treated with pervanadate
- whole cell lysate provided as Western blotting positive control
- should be stored at -20°C and repeated freezing and thawing should be minimized
- sample vial should be placed at 95° C for up to 5 minutes, once prior to use
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The A-431 + pervanadate cell lysate originates from the A-431 cell line, an epidermoid carcinoma derived from an older adult. This particular lysate is enhanced by the addition of pervanadate, a potent inhibitor of protein tyrosine phosphatases. Pervanadate acts by mimicking phosphotyrosine, thus blocking the dephosphorylation process, leading to an increase in phosphorylated proteins within the cell. This modification is particularly useful for studying tyrosine phosphorylation events, which are crucial in many signaling pathways, including those related to cell growth and apoptosis. The A-431 cells, known for their robust expression of the epidermal growth factor receptor (EGFR), provide a dynamic system for examining how perturbations in phosphorylation can affect cellular processes. Researchers utilize the A-431 + pervanadate cell lysate to investigate the roles of phosphorylated proteins in various biological contexts, such as signal transduction, cellular communication, and protein network dynamics. By maintaining proteins in their phosphorylated state, this lysate allows for deeper insights into the mechanisms by which cells regulate these critical proteins, offering valuable information for basic research in cellular and molecular biology.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
A-431 + pervanadate Cell Lysate | sc-24654 | 500 µg/200 µl | $118.00 |