SK-BR-3 + PMA Cell Lysate: sc-24773

SK-BR-3 + PMA cell lysate comes from the SK-BR-3 human breast cancer cell line treated with phorbol 12-myristate 13-acetate (PMA), a potent activator of protein kinase C (PKC). This treatment is widely used in research to explore PKC-mediated signaling pathways in cancer cells. In SK-BR-3 cells, which overexpress the HER2/neu protein, PMA exposure typically leads to enhanced activation of downstream signaling cascades, such as the MAPK and NF-κB pathways, which are involved in cellular proliferation and survival. Researchers utilize this lysate to investigate how PKC activation affects these pathways specifically in the context of HER2-positive breast cancer cells. This includes studies on gene expression changes, cellular responses to oxidative stress, and alterations in apoptotic pathways. Additionally, SK-BR-3 + PMA cell lysate is critical for examining the cross-talk between PKC and other signaling molecules, offering insights into the complex network of intracellular signals that regulate cancer cell behavior. The application of this lysate in research settings allows for a detailed analysis of the molecular mechanisms by which PKC influences cell fate decisions in cancer cells, significantly contributing to basic cellular and molecular biology.