U-937 Cell Lysate: sc-2239
- 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
- human whole cell lysate; histiocytic lymphoma cells
- whole cell lysate provided as Western blotting positive control
- should be stored at -20°C and repeated freezing and thawing should be minimized
- sample vial should be placed at 95° C for up to 5 minutes, once prior to use
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U-937 Cell Lysate is derived from U-937 cells, a human histiocytic lymphoma cell line originally established from the pleural effusion of a 37-year-old male patient with diffuse histiocytic lymphoma. This lysate is extensively used in immunological and oncological research to study monocyte and macrophage differentiation, signal transduction, and the mechanisms of leukemia and lymphoma. U-937 cells can be induced to differentiate into macrophage-like cells, making the lysate valuable for investigating the molecular changes associated with differentiation and activation. Researchers utilize U-937 Cell Lysate to analyze the expression and post-translational modifications of key proteins involved in signaling pathways such as NF-κB, JAK/STAT, and PI3K/AKT using techniques like western blotting, immunoprecipitation, and mass spectrometry. This lysate is also instrumental in studying the effects of various cytokines and chemical agents on cell signaling and gene expression. Additionally, U-937 Cell Lysate aids in the identification of protein interactions and the elucidation of pathways that regulate apoptosis, proliferation, and immune responses. By comparing U-937 lysates with those from normal monocytes and macrophages, scientists can uncover specific molecular alterations associated with histiocytic lymphoma, providing insights into the cellular mechanisms driving malignancy and immune regulation.
U-937 Cell Lysate References:
- Role for caspase-mediated cleavage of Rad51 in induction of apoptosis by DNA damage. | Huang, Y., et al. 1999. Mol Cell Biol. 19: 2986-97. PMID: 10082566
- Functional interaction between SHPTP1 and the Lyn tyrosine kinase in the apoptotic response to DNA damage. | Yoshida, K., et al. 1999. J Biol Chem. 274: 34663-8. PMID: 10574931
- Hypoxia-activated ligand HAL-1/13 is lupus autoantigen Ku80 and mediates lymphoid cell adhesion in vitro. | Lynch, EM., et al. 2001. Am J Physiol Cell Physiol. 280: C897-911. PMID: 11245607
- Statins and PPARalpha agonists induce myotoxicity in differentiated rat skeletal muscle cultures but do not exhibit synergy with co-treatment. | Johnson, TE., et al. 2005. Toxicol Appl Pharmacol. 208: 210-21. PMID: 16239165
- Sex differences in PPARgamma expressions in rat adipose tissues. | Kadowaki, K., et al. 2007. Biol Pharm Bull. 30: 818-20. PMID: 17409529
- The effect of sex hormones on peroxisome proliferator-activated receptor gamma expression and activity in mature adipocytes. | Sato, H., et al. 2013. Biol Pharm Bull. 36: 564-73. PMID: 23546292
- Protective activity of carnosine and anserine against zinc-induced neurotoxicity: a possible treatment for vascular dementia. | Mizuno, D., et al. 2015. Metallomics. 7: 1233-9. PMID: 25846004
- Mammalian protein secretion without signal peptide removal. Biosynthesis of plasminogen activator inhibitor-2 in U-937 cells. | Ye, RD., et al. 1988. J Biol Chem. 263: 4869-75. PMID: 3127394
- The p60 tumor necrosis factor (TNF) receptor-associated kinase (TRAK) binds residues 344-397 within the cytoplasmic domain involved in TNF signaling. | Darnay, BG., et al. 1995. J Biol Chem. 270: 14867-70. PMID: 7797464
- Identification of a protein kinase associated with the cytoplasmic domain of the p60 tumor necrosis factor receptor. | Darnay, BG., et al. 1994. J Biol Chem. 269: 20299-304. PMID: 8051124
- Moesin: a cell membrane protein linked with susceptibility to measles virus infection. | Dunster, LM., et al. 1994. Virology. 198: 265-74. PMID: 8259662
- Inhibition of phosphatidylinositol 3-kinase by c-Abl in the genotoxic stress response. | Yuan, ZM., et al. 1997. J Biol Chem. 272: 23485-8. PMID: 9295282
- Regulation of Rad51 function by c-Abl in response to DNA damage. | Yuan, ZM., et al. 1998. J Biol Chem. 273: 3799-802. PMID: 9461559
- Regulation of DNA-dependent protein kinase by the Lyn tyrosine kinase. | Kumar, S., et al. 1998. J Biol Chem. 273: 25654-8. PMID: 9748231
- Inactivation of DNA-dependent protein kinase by protein kinase Cdelta: implications for apoptosis. | Bharti, A., et al. 1998. Mol Cell Biol. 18: 6719-28. PMID: 9774685
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
U-937 Cell Lysate | sc-2239 | 500 µg/200 µl | $118.00 |