MCF7 Whole Cell Lysate whole cell lysate provided as Western blotting positive control

MCF7 Whole Cell Lysate: sc-2206

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Datasheets

    • 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
    • human whole cell lysate; mammary adenocarcinoma cells
    • whole cell lysate provided as Western blotting positive control
    • should be stored at -20°C and repeated freezing and thawing should be minimized
    • sample vial should be boiled once prior to use

Does MCF7 cell lysate we use in Imunohistochemistry as positive control ? If we prepare paraffin embadded slide from MCF7 cell lysate??

Asked by: Mahmud
Thank you for your question. You can't embed cells from a cell lysate in paraffin, but you can use MCF-7 cells as a positive controls in IF or immunocytochemistry. I would suggest fixing in pfa, acetone or methanol instead of formalin. You can find our IF and IHC protocols here: https://www.scbt.com/resources/protocols/immunofluorescence-cell-staining https://www.scbt.com/resources/protocols/immunoperoxidase-staining
Answered by: Tech Service
Date published: 2020-02-13
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