AtT-20/D16vF2 Whole Cell Lysate: sc-364367

The AtT-20/D16vF2 whole cell lysate is derived from a murine pituitary tumor cell line that has been extensively utilized in research settings, particularly in the field of endocrinology and cellular biology. This lysate, which contains a complex mixture of cellular components including proteins, DNA, RNA, and various metabolites, is prepared by lysing AtT-20/D16vF2 cells, which are known for their ability to produce and secrete pro-opiomelanocortin (POMC) derivatives such as ACTH (adrenocorticotropic hormone). Originating from a corticotroph tumor of the anterior pituitary, the AtT-20/D16vF2 cell line serves as an exemplary model for studying the regulation of peptide hormone secretion and the intracellular signaling pathways involved in neuroendocrine processes. Researchers utilize this lysate to investigate gene expression patterns, protein interactions, and the effects of genetic or environmental modifications on cellular functions. The use of whole cell lysates like that from AtT-20/D16vF2 enables a comprehensive analysis of cellular components, providing insights into the molecular mechanisms that underpin various biological processes and responses. As such, this lysate is a valuable resource for advancing our understanding of cell biology in a controlled, experimental context.