Jurkat + PMA Cell Lysate: sc-24718

Jurkat + PMA Cell Lysate is derived from Jurkat cells, a human T-cell leukemia line, treated with Phorbol 12-myristate 13-acetate (PMA), a potent activator of protein kinase C (PKC). This lysate captures the cellular biochemical environment following PMA exposure, which is used to mimic aspects of T-cell activation. PMA's interaction with PKC plays a crucial role in modulating various signaling pathways within the cell, particularly those associated with cell proliferation, differentiation, and secretion of cytokines. The lysate contains a diverse mix of cellular components, including proteins, enzymes, and nucleic acids, providing a snapshot of cellular activity during heightened PKC activity. Researchers use Jurkat + PMA Cell Lysate extensively in studies aimed at understanding T-cell biology, especially the intracellular signaling cascades triggered during immune responses. By analyzing this lysate, researchers can investigate the downstream effects of PKC activation, such as changes in gene expression, activation of transcription factors, and the initiation of cellular responses to external stimuli. This lysate is thus a valuable tool in research focused on elucidating the mechanisms of cellular signaling in immunology and the molecular events that govern T-cell function and behavior under stimulated conditions.