NIH/3T3 + UV Cell Lysate: sc-3804

NIH/3T3 + UV cell lysate is derived from NIH/3T3 cells, a mouse embryonic fibroblast cell line, following exposure to ultraviolet (UV) radiation. This lysate is extensively utilized in scientific research to study DNA damage responses, cellular stress mechanisms, and apoptosis. UV radiation induces DNA lesions such as cyclobutane pyrimidine dimers (CPDs) and 6-4 photoproducts, triggering cellular repair mechanisms. Researchers employ NIH/3T3 + UV cell lysate to investigate the activation of key signaling pathways, including the p53 pathway, which plays a crucial role in cell cycle arrest and apoptosis in response to DNA damage. This lysate is instrumental in studying protein expression and post-translational modifications like phosphorylation of proteins involved in DNA repair and stress response, such as ATM, ATR, Chk1, and Chk2 kinases. Additionally, it allows for the examination of UV-induced activation of transcription factors like NF-κB and AP-1, which regulate genes involved in inflammation and cell survival. NIH/3T3 + UV cell lysate is also used to assess the effects of various chemical modulators on DNA repair pathways and apoptosis. Recent studies have leveraged this lysate for proteomic and genomic analyses to identify novel proteins and signaling networks involved in the UV-induced stress response. This lysate remains a valuable resource for advancing our understanding of cellular mechanisms related to DNA damage and repair.