NIH/3T3 + serum Cell Lysate: sc-2248

NIH/3T3 + serum cell lysate is derived from NIH/3T3 cells, a mouse embryonic fibroblast cell line, following stimulation with serum. This lysate is extensively used in research to study cellular signaling, growth regulation, and response to mitogenic stimuli. Serum contains a complex mixture of growth factors, hormones, and nutrients that stimulate cell proliferation and survival. Researchers employ NIH/3T3 + serum cell lysate to investigate the activation of key signaling pathways, such as the PI3K/Akt, Ras-MAPK/ERK, and mTOR pathways, which are critical for regulating cell growth, metabolism, and survival. This lysate facilitates the study of protein expression and post-translational modifications, including phosphorylation of proteins involved in these signaling cascades. Additionally, it allows for the examination of serum-induced activation of transcription factors like MYC and NF-κB, which regulate genes essential for cell cycle progression and proliferation. NIH/3T3 + serum cell lysate is also used to assess the effects of various chemical modulators on growth factor signaling pathways, providing insights into the molecular mechanisms of cell proliferation and survival. Recent research has utilized this lysate for proteomic and transcriptomic analyses to identify novel proteins and signaling networks involved in serum-induced cellular responses. This lysate remains a valuable resource for advancing our understanding of cell biology and growth regulation.