PC-12 + PMA Cell Lysate: sc-24757

PC-12 + PMA cell lysate is derived from PC-12 cells, a rat pheochromocytoma cell line, treated with phorbol 12-myristate 13-acetate (PMA). This lysate is a pivotal research tool for studying the mechanisms of signal transduction and cellular differentiation. PMA activates protein kinase C (PKC), which plays a critical role in various cellular processes, including differentiation, proliferation, and gene expression. Researchers use PC-12 + PMA cell lysate to investigate the PKC-mediated pathways and their downstream effects on the mitogen-activated protein kinase (MAPK) pathway, particularly the ERK1/2 signaling cascade. This lysate enables the study of phosphorylation events and the activation of transcription factors such as AP-1 and NF-κB, which are essential for regulating gene expression in response to extracellular signals. Additionally, it is used to analyze the interplay between PKC and other signaling molecules, including PI3K/Akt and JNK pathways. Recent research has utilized this lysate in proteomic studies to identify PKC substrates and to understand the broader network of signaling interactions. By providing a detailed understanding of PKC's role in cellular signaling, PC-12 + PMA cell lysate remains a valuable asset for elucidating complex biochemical pathways in neurobiological and cellular studies.