THP-1 Cell Lysate: sc-2238
- 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
- human whole cell lysate; monocytic leukemia cells
- whole cell lysate provided as Western blotting positive control
- should be stored at -20°C and repeated freezing and thawing should be minimized
- sample vial should be placed at 95° C for up to 5 minutes, once prior to use
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THP-1 Cell Lysate is derived from THP-1 cells, a human monocytic cell line established from the peripheral blood of a 1-year-old male with acute monocytic leukemia. This lysate is extensively used in immunological and hematological research to study monocyte and macrophage biology, signaling pathways, and the molecular mechanisms of leukemia. THP-1 cells can be differentiated into macrophage-like cells in response to various stimuli, making the lysate valuable for investigating changes in protein expression, phosphorylation, and interactions during differentiation and activation. Researchers utilize THP-1 Cell Lysate to analyze key signaling pathways such as NF-κB, MAPK/ERK, and PI3K/AKT using techniques like western blotting, immunoprecipitation, and mass spectrometry. This lysate is particularly useful for studying the effects of cytokines, chemokines, and other immune modulators on monocyte activation and function. Additionally, it aids in exploring the molecular basis of inflammatory responses, phagocytosis, and antigen presentation by examining the roles of surface receptors, transcription factors, and cytokine production. By comparing THP-1 lysates with those from normal monocytes, scientists can identify specific molecular alterations associated with leukemia and immune responses. This research advances our understanding of monocyte biology and the underlying mechanisms of immune regulation and hematological malignancies.
THP-1 Cell Lysate References:
- Opposing cytokine-specific effects of all trans-retinoic acid on the activation and expression of signal transducer and activator of transcription (STAT)-1 in THP-1 cells. | Chen, Q., et al. 2002. Immunology. 107: 199-208. PMID: 12383199
- Amlodipine modulates THP-1 cell adhesion to vascular endothelium via inhibition of protein kinase C signal transduction. | Yu, T., et al. 2003. Hypertension. 42: 329-34. PMID: 12900427
- Activation of hypoxia-inducible factor 1 during macrophage differentiation. | Oda, T., et al. 2006. Am J Physiol Cell Physiol. 291: C104-13. PMID: 16481368
- Myosin phosphatase interacts with and dephosphorylates the retinoblastoma protein in THP-1 leukemic cells: its inhibition is involved in the attenuation of daunorubicin-induced cell death by calyculin-A. | Kiss, A., et al. 2008. Cell Signal. 20: 2059-70. PMID: 18755268
- Inhibition of carboxylesterase 1 is associated with cholesteryl ester retention in human THP-1 monocyte/macrophages. | Crow, JA., et al. 2008. Biochim Biophys Acta. 1781: 643-54. PMID: 18762277
- Dynamic expression of peptidylarginine deiminase 2 in human monocytic leukaemia THP-1 cells during macrophage differentiation. | Hojo-Nakashima, I., et al. 2009. J Biochem. 146: 471-9. PMID: 19564157
- Syk protein tyrosine kinase involves PECAM-1 signaling through tandem immunotyrosine inhibitory motifs in human THP-1 macrophages. | Wang, J., et al. 2011. Cell Immunol. 272: 39-44. PMID: 22000807
- Cytosolic, autocrine alpha-1 proteinase inhibitor (A1PI) inhibits caspase-1 and blocks IL-1β dependent cytokine release in monocytes. | Wang, Y., et al. 2012. PLoS One. 7: e51078. PMID: 23226468
- Evaluation of amentoflavone isolated from Cnestis ferruginea Vahl ex DC (Connaraceae) on production of inflammatory mediators in LPS stimulated rat astrocytoma cell line (C6) and THP-1 cells. | Ishola, IO., et al. 2013. J Ethnopharmacol. 146: 440-8. PMID: 23376104
- Virtual Screening of Some Active Human Macrophage Migration Inhibitory Factor Antagonists. | Tsai, TL. and Lin, TH. 2014. J Biomol Screen. 19: 1116-23. PMID: 24554444
- Observation of glycolytic metabolites in tumor cell lysate by using hyperpolarization of deuterated glucose. | Kumagai, K., et al. 2014. Biol Pharm Bull. 37: 1416-21. PMID: 25087964
- NLRP3 Inflammasome Activation in THP-1 Target Cells Triggered by Pathogenic Naegleria fowleri. | Kim, JH., et al. 2016. Infect Immun. 84: 2422-8. PMID: 27297387
- Semi-quantification of antibody-dependent enhancement (ADE) in the uptake of Adenovirus serotype 5 into THP-1 cells. | Valliyott, L., et al. 2020. Anal Biochem. 591: 113568. PMID: 31881180
- Distinct anti-proliferative effects of herbal melanin on human acute monocytic leukemia THP-1 cells and embryonic kidney HEK293 cells. | El-Obeid, A., et al. 2020. BMC Complement Med Ther. 20: 154. PMID: 32448225
- Investigating the Effects of Biogenic Zinc Oxide Nanoparticles Produced Using Papaver somniferum Extract on Oxidative Stress, Cytotoxicity, and the Induction of Apoptosis in the THP-1 Cell Line. | Kadhim, AA., et al. 2023. Biol Trace Elem Res.. PMID: 36662347
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
THP-1 Cell Lysate | sc-2238 | 500 µg/200 µl | $118.00 |