CCRF-CEM Cell Lysate: sc-2225

The CCRF-CEM Cell Lysate is derived from the CCRF-CEM cell line, a line of human T lymphoblasts originally isolated from a patient with acute lymphoblastic leukemia. This lysate encompasses a rich array of cellular contents, including proteins, nucleic acids, and other crucial cellular constituents, obtained through thoroughgoing methods such as detergent lysis, sonication, or enzymatic disruption. Its origin from T lymphoblasts makes the CCRF-CEM Cell Lysate an invaluable resource in biomedical research, primarily focused on understanding cellular mechanisms in non-medical contexts. Researchers utilize this lysate to investigate fundamental processes such as cell cycle progression, apoptosis, and cellular signaling pathways particularly relevant to lymphoblastic cells. The CCRF-CEM Cell Lysate offers insights into the regulation of gene expression, protein synthesis, and the interaction between cellular pathways that drive T cell development and function. It is also extensively used in proteomics for identifying and characterizing protein interactions and modifications, which are essential for understanding the intracellular dynamics that underpin cellular responses to various stimuli. Studies using this lysate significantly contribute to the field of cell biology by elucidating the complex networks within cells that maintain immune function and cellular integrity in research-focused explorations.

CCRF-CEM Cell Lysate References:

1. The role of AP-1 in glucocorticoid resistance in leukaemia.  |  Bailey, S., et al. 2001. Leukemia. 15: 391-7. PMID: 11237062
2. Submitochondrial localization of the mitochondrial isoform of folylpolyglutamate synthetase in CCRF-CEM human T-lymphoblastic leukemia cells.  |  Nair, JR. and McGuire, JJ. 2005. Biochim Biophys Acta. 1746: 38-44. PMID: 16169100
3. Micellar electrokinetic capillary chromatography reveals differences in intracellular metabolism between liposomal and free doxorubicin treatment of human leukemia cells.  |  Eder, AR. and Arriaga, EA. 2005. J Chromatogr B Analyt Technol Biomed Life Sci. 829: 115-22. PMID: 16246643
4. Novel potent inhibitors of deoxycytidine kinase identified and compared by multiple assays.  |  Yu, XC., et al. 2010. J Biomol Screen. 15: 72-9. PMID: 19959816
5. Visual detection of microRNA with lateral flow nucleic acid biosensor.  |  Gao, X., et al. 2014. Biosens Bioelectron. 54: 578-84. PMID: 24333569
6. Protein phosphatase 2A regulates deoxycytidine kinase activity via Ser-74 dephosphorylation.  |  Amsailale, R., et al. 2014. FEBS Lett. 588: 727-32. PMID: 24462681
7. Cancer biomarker discovery using DNA aptamers.  |  Jin, C., et al. 2016. Analyst. 141: 461-6. PMID: 26567694
8. DNA micelle flares: a study of the basic properties that contribute to enhanced stability and binding affinity in complex biological systems.  |  Wang, Y., et al. 2016. Chem Sci. 7: 6041-6049. PMID: 28066539
9. Fluorinated molecular beacons as functional DNA nanomolecules for cellular imaging.  |  Jin, C., et al. 2017. Chem Sci. 8: 7082-7086. PMID: 29147537
10. SELEX methods on the road to protein targeting with nucleic acid aptamers.  |  Bayat, P., et al. 2018. Biochimie. 154: 132-155. PMID: 30193856
11. Methadone hydrochloride and leukemia cells: Effects on cell viability, DNA fragmentation and apoptotic proteins expression level.  |  Kua, VMD., et al. 2019. Pak J Pharm Sci. 32: 1797-1803. PMID: 31680075
12. Separation and comparison of human TL-like antigens and HLA(A, B, C) antigens expressed on cultured T cells.  |  Tokuyama, H. and Tanigaki, N. 1981. Immunogenetics. 13: 147-65. PMID: 6164635