Ramos Cell Lysate: sc-2216

Ramos Cell Lysate originates from the Ramos cell line, which is a human Burkitt's lymphoma line extensively used in immunological research. This lysate provides a complex mixture of cellular contents, which includes proteins involved in multiple signaling pathways, particularly those relevant to B-cell development and function. The lysate is frequently utilized in the study of protein interactions and signaling mechanisms within B-cells, such as the B-cell receptor (BCR) signaling pathway, crucial for B-cell activation and proliferation. Researchers employ this lysate in techniques like Western blotting and immunoprecipitation to investigate post-translational modifications and protein-protein interactions that are not readily discernible in less complex systems. Additionally, it has been instrumental in exploring the role of NF-κB, a key transcription factor in the immune response, by allowing scientists to examine its activation and the subsequent effect on gene expression. The Ramos Cell Lysate is particularly valuable for its ability to mimic native cellular conditions, thereby providing deeper insights into the molecular underpinnings of B-cell biology and lymphoma pathogenesis.

Ramos Cell Lysate References:

1. Uptake and activation of eicosapentaenoic acid are related to accumulation of triacylglycerol in Ramos cells dying from apoptosis.  |  Finstad, HS., et al. 2000. J Lipid Res. 41: 554-63. PMID: 10744776
2. Globotriaosyl ceramide (CD77/Gb3) in the glycolipid-enriched membrane domain participates in B-cell receptor-mediated apoptosis by regulating lyn kinase activity in human B cells.  |  Mori, T., et al. 2000. Exp Hematol. 28: 1260-8. PMID: 11063874
3. Characterization of molecular components associated with surface immunoglobulin M in human B lymphocytes: presence of tyrosine and serine/threonine protein kinases.  |  Leprince, C., et al. 1992. Eur J Immunol. 22: 2093-9. PMID: 1386314
4. Kinetic assay for characterization of spleen tyrosine kinase activity and inhibition with recombinant kinase and crude cell lysates.  |  Li, M., et al. 2009. Anal Biochem. 384: 56-67. PMID: 18762159
5. Localization of Toll-like receptors on epididymal epithelial cells and spermatozoa.  |  Palladino, MA., et al. 2008. Am J Reprod Immunol. 60: 541-55. PMID: 19032616
6. Characterization of clathrin and Syk interaction upon Shiga toxin binding.  |  Wälchli, S., et al. 2009. Cell Signal. 21: 1161-8. PMID: 19289168
7. A bioresponsive controlled-release biosensor using Au nanocages capped with an aptamer-based molecular gate and its application in living cells.  |  Wang, W., et al. 2012. Chem Commun (Camb). 48: 10228-30. PMID: 22968197
8. Development of a pharmacodynamic assay based on PLCγ2 phosphorylation for quantifying spleen tyrosine kinase (SYK)-Bruton′s tyrosine kinase (BTK) signaling.  |  Hsu, J., et al. 2013. J Biomol Screen. 18: 890-8. PMID: 23704133
9. Nuclear factor of activated T-cell activity is associated with metastatic capacity in colon cancer.  |  Tripathi, MK., et al. 2014. Cancer Res. 74: 6947-57. PMID: 25320007
10. Isothermal amplified detection of ATP using Au nanocages capped with a DNA molecular gate and its application in cell lysates.  |  Wang, W., et al. 2015. Analyst. 140: 1672-7. PMID: 25627025
11. A bioresponsive controlled-release bioassay based on aptamer-gated Au nanocages and its application in living cells.  |  Wang, W., et al. 2015. Chem Commun (Camb). 51: 9109-12. PMID: 25939588
12. Direct detection of nitrotyrosine-containing proteins using an aniline-based oxidative coupling strategy.  |  Sangsuwan, R., et al. 2016. Chem Commun (Camb). 52: 10036-9. PMID: 27447346
13. Exosomal MicroRNAs in Milk from Mothers Delivering Preterm Infants Survive in Vitro Digestion and Are Taken Up by Human Intestinal Cells.  |  Kahn, S., et al. 2018. Mol Nutr Food Res. 62: e1701050. PMID: 29644801