Jurkat Whole Cell Lysate: sc-2204
- 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
- human whole cell lysate; acute T cell leukemia cells
- whole cell lysate provided as Western blotting positive control
- should be stored at -20°C and repeated freezing and thawing should be minimized
- sample vial should be placed at 95° C for up to 5 minutes, once prior to use
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Jurkat Whole Cell Lysate is derived from Jurkat cells, an immortalized line of human T lymphocytes frequently used in immunological and cellular biology research. These cells originate from an acute T cell leukemia and are widely recognized for their ability to provide consistent and reproducible data in various experimental setups. The whole cell lysate from Jurkat cells contains a comprehensive array of cellular components, including proteins, RNA, DNA, lipids, and metabolites, reflecting the entirety of the cell's constituents. This lysate is particularly valuable for researchers looking to study T cell behavior under various conditions, as it allows for the examination of complete cellular responses without the need to focus on specific subcellular fractions. Jurkat Whole Cell Lysate is extensively used in applications such as Western blotting, mass spectrometry, and proteomic analysis to identify and quantify proteins, study post-translational modifications, and investigate protein-protein interactions. Additionally, it serves as a critical tool in the study of signal transduction pathways, especially those related to apoptosis, activation, and cellular stress responses. By providing a holistic view of the cellular architecture and molecular machinery, Jurkat Whole Cell Lysate enables a deeper understanding of T cell physiology and the complex network of signals that regulate immune responses.
Jurkat Whole Cell Lysate References:
- Peptides derived from BH3 domains of Bcl-2 family members: a comparative analysis of inhibition of Bcl-2, Bcl-x(L) and Bax oligomerization, induction of cytochrome c release, and activation of cell death. | Shangary, S. and Johnson, DE. 2002. Biochemistry. 41: 9485-95. PMID: 12135371
- Oxidative stress is associated with increased apoptosis leading to spermatozoa DNA damage in patients with male factor infertility. | Wang, X., et al. 2003. Fertil Steril. 80: 531-5. PMID: 12969693
- Subcellular localization of ALMS1 supports involvement of centrosome and basal body dysfunction in the pathogenesis of obesity, insulin resistance, and type 2 diabetes. | Hearn, T., et al. 2005. Diabetes. 54: 1581-7. PMID: 15855349
- A complex of Shc and Ran-GTPase localises to the cell nucleus. | George, R., et al. 2009. Cell Mol Life Sci. 66: 711-20. PMID: 19153664
- Isotopic signature transfer and mass pattern prediction (IsoStamp): an enabling technique for chemically-directed proteomics. | Palaniappan, KK., et al. 2011. ACS Chem Biol. 6: 829-36. PMID: 21604797
- Development and application of an in vitro apoptin kinase assay. | Lanz, HL., et al. 2012. Anal Biochem. 421: 68-74. PMID: 22080040
- Detection and quantification of farnesol-induced apoptosis in difficult primary cell cultures by TaqMan protein assay. | Pfister, C., et al. 2013. Apoptosis. 18: 452-66. PMID: 23315006
- The presence of a truncated base excision repair pathway in human spermatozoa that is mediated by OGG1. | Smith, TB., et al. 2013. J Cell Sci. 126: 1488-97. PMID: 23378024
- Nuclear lymphocyte-specific protein tyrosine kinase and its interaction with CR6-interacting factor 1 promote the survival of human leukemic T cells. | Vahedi, S., et al. 2015. Oncol Rep. 34: 43-50. PMID: 25997448
- Development of IsoTaG, a Chemical Glycoproteomics Technique for Profiling Intact N- and O-Glycopeptides from Whole Cell Proteomes. | Woo, CM., et al. 2017. J Proteome Res. 16: 1706-1718. PMID: 28244757
- Novel chemiluminescent Western blot blocking and antibody incubation solution for enhanced antibody-antigen interaction and increased specificity. | Schwartz, K. and Bochkariov, D. 2017. Electrophoresis. 38: 2631-2637. PMID: 28704589
- Expression of Angiopoietins and Angiogenic Signaling Pathway Molecules in Chronic Subdural Hematomas. | Isaji, T., et al. 2020. J Neurotrauma. 37: 2493-2498. PMID: 32458767
- Requirement for the coexpression of T3 and the T cell antigen receptor on a malignant human T cell line. | Weiss, A. and Stobo, JD. 1984. J Exp Med. 160: 1284-99. PMID: 6208306
- ′Spot transfer′, elution and comigration with known proteins allows accurate transferral of protein identifications between distinct two-dimensional electrophoretic systems. | Dean, DP., et al. 1994. Electrophoresis. 15: 540-3. PMID: 8055881
- CD28 of T lymphocytes associates with phosphatidylinositol 3-kinase. | August, A. and Dupont, B. 1994. Int Immunol. 6: 769-74. PMID: 8080844
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Jurkat Whole Cell Lysate | sc-2204 | 500 µg/200 µl | $118.00 |