C3H/10T1/2 Cell Lysate: sc-3801
- 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
- mouse whole cell lysate; contact sensitive embryo cells
- whole cell lysate provided as Western blotting positive control
- should be stored at -20°C and repeated freezing and thawing should be minimized
- sample vial should be placed at 95° C for up to 5 minutes, once prior to use
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SEE ALSO...
The C3H/10T1/2 Cell Lysate is produced from the C3H/10T1/2 cell line, a murine fibroblast line derived from C3H mouse embryos. This lysate contains a comprehensive array of cellular components, including proteins, nucleic acids, and other molecular entities, extracted through rigorous cell lysis procedures such as ultrasonication, freeze-thaw cycles, or detergent-based lysis. This particular cell line, and consequently the lysate, has been widely utilized in research focusing on the cellular mechanisms underpinning differentiation and transformation. The versatility of the C3H/10T1/2 lysate makes it an invaluable resource in the study of cellular signaling pathways and gene expression, especially in contexts simulating various environmental or genetic modifications. It is frequently employed as a control or standard in biochemical assays and protein profiling, where understanding the baseline levels of various biomolecules is crucial. Additionally, the lysate is a key tool in developmental biology, used to investigate the factors that influence cell differentiation and to monitor the cellular impacts of genetic manipulation. By providing a detailed biochemical snapshot of fibroblast cells, the C3H/10T1/2 Cell Lysate aids in the exploration of fundamental biological processes, contributing to a deeper understanding of cellular behavior.
C3H/10T1/2 Cell Lysate References:
- Cortactin localization to sites of actin assembly in lamellipodia requires interactions with F-actin and the Arp2/3 complex. | Weed, SA., et al. 2000. J Cell Biol. 151: 29-40. PMID: 11018051
- Activation of the renin-angiotensin system within podocytes in diabetes. | Yoo, TH., et al. 2007. Kidney Int. 71: 1019-27. PMID: 17361112
- Gene- and Species-Specific Hox mRNA Translation by Ribosome Expansion Segments. | Leppek, K., et al. 2020. Mol Cell. 80: 980-995.e13. PMID: 33202249
- Evaluation of BMP2/miRNA co-expression systems for potent therapeutic efficacy in bone-tissue regeneration. | Brenner, TK., et al. 2021. Eur Cell Mater. 41: 245-268. PMID: 33660785
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C3H/10T1/2 Cell Lysate | sc-3801 | 500 µg/200 µl | $118.00 |