RAW 264.7 + LPS/PMA Cell Lysate: sc-2212
- 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
- mouse whole cell lysate; Abelson transformed macrophage cells induced with LPS and PMA
- whole cell lysate provided as Western blotting positive control
- should be stored at -20°C and repeated freezing and thawing should be minimized
- sample vial should be placed at 95° C for up to 5 minutes, once prior to use
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RAW 264.7 + LPS/PMA cell lysate is derived from the RAW 264.7 murine macrophage cell line treated with lipopolysaccharide (LPS) and phorbol 12-myristate 13-acetate (PMA). This lysate is invaluable for investigating the combined impact of these agents on macrophage activation and signaling pathways. LPS, a bacterial endotoxin, engages toll-like receptor 4 (TLR4) to initiate a cascade that activates NF-κB, leading to an increase in pro-inflammatory cytokine production. Simultaneously, PMA activates protein kinase C (PKC), influencing pathways like MAPK, which are crucial for further inflammatory mediator production. Researchers use this lysate to study the synergistic effects of LPS and PMA on macrophage behavior, focusing on changes in cytokine profiles, upregulation of co-stimulatory molecules, and enhanced production of reactive oxygen species. This comprehensive approach allows for a deeper understanding of macrophage response mechanisms under complex stimulation conditions.
RAW 264.7 + LPS/PMA Cell Lysate References:
- Cyclooxygenase in normal human tissues--is COX-1 really a constitutive isoform, and COX-2 an inducible isoform? | Zidar, N., et al. 2009. J Cell Mol Med. 13: 3753-63. PMID: 18657230
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RAW 264.7 + LPS/PMA Cell Lysate | sc-2212 | 500 µg/200 µl | $118.00 |