NIH/3T3 Cell Lysate for Immunoprecipaitation: sc-24794

NIH/3T3 (IP) cell lysate is derived from NIH/3T3 cells, a mouse embryonic fibroblast cell line, after immunoprecipitation (IP) of specific proteins or protein complexes. This lysate is extensively used in scientific research to investigate protein-protein interactions, signaling pathways, and cellular mechanisms. The NIH/3T3 cell line is characterized by its stable growth and susceptibility to oncogenic transformation, making it a valuable model for studying cell cycle regulation, proliferation, and differentiation. Researchers use NIH/3T3 (IP) cell lysate to analyze key signaling pathways, including the Ras-MAPK/ERK, PI3K/Akt, and JAK/STAT pathways, which are critical for cell growth and survival. The IP process allows for the isolation and detailed study of specific proteins and their associated complexes, facilitating the identification of binding partners and regulatory mechanisms. This lysate is instrumental in examining post-translational modifications, such as phosphorylation, ubiquitination, and acetylation, that modulate protein function and interactions. Additionally, NIH/3T3 (IP) cell lysate is used to assess the impact of various chemical modulators on these signaling pathways, providing insights into cellular responses and mechanisms of action. Recent research has leveraged this lysate for proteomic analyses to identify novel signaling networks and regulatory proteins involved in cellular processes. NIH/3T3 (IP) cell lysate continues to be a crucial resource for advancing our understanding of cell biology and molecular signaling.