RAW 264.7 + LPS/IFN-γ Cell Lysate: sc-24767

The RAW 264.7 + LPS/IFN-γ cell lysate is derived from the RAW 264.7 cell line, a murine macrophage model widely utilized to explore the nuances of innate immune responses and macrophage activation. In this specific lysate, cells are stimulated with lipopolysaccharide (LPS) and interferon-gamma (IFN-γ), a combination that synergistically promotes a highly activated macrophage state. LPS, a potent activator of the Toll-like receptor 4 (TLR4), triggers a cascade of intracellular signaling that enhances the transcription of pro-inflammatory genes primarily through the NF-κB pathway. Meanwhile, IFN-γ, a critical cytokine in innate and adaptive immunity, further amplifies this response by activating the STAT1 pathway, which not only boosts the expression of antimicrobial effector mechanisms but also upregulates major histocompatibility complex (MHC) molecules, enhancing antigen presentation. This specific lysate model is instrumental in studying the cross-talk between TLR and cytokine signaling pathways, providing deep insights into the mechanisms by which macrophages orchestrate immune responses. Researchers utilize this lysate to dissect cellular processes such as cytokine production, reactive oxygen species generation, and gene expression regulation under conditions simulating an immune challenge.

RAW 264.7 + LPS/IFN-γ Cell Lysate References:

1. Modulation of ENaC, CFTR, and iNOS expression in bronchial epithelial cells after stimulation with Staphylococcus epidermidis (94B080) and Staphylococcus aureus (90B083).  |  Hussain, R., et al. 2013. APMIS. 121: 814-26. PMID: 23879620