GH3 Whole Cell Lysate: sc-364777

GH3 whole cell lysate is derived from the GH3 cell line, originally established from rat pituitary tumor cells. This cell line is extensively used in endocrinological and cancer research to study the regulation of hormone secretion and the cellular mechanisms underlying pituitary adenomas. GH3 cells are known for their ability to produce and secrete prolactin and growth hormone, making the lysate particularly valuable for investigating the signaling pathways involved in hormone regulation, such as the cAMP/PKA pathway and the effects of thyroid hormones. Researchers utilize GH3 cell lysate to examine the expression of hormone receptors and the intracellular signaling cascades triggered upon hormone binding. Additionally, this lysate is instrumental in studying the impact of various pharmacological agents on pituitary cell function and proliferation, providing insights into the cellular responses to drugs and potential endocrine disruptors. The GH3 lysate aids in exploring gene expression patterns and post-translational modifications that regulate hormone synthesis and release, contributing significantly to our understanding of pituitary gland physiology and pathology.

GH3 Whole Cell Lysate References:

1. Tissue-specific differential repression of gene expression by a dominant negative mutant of thyroid hormone beta1 receptor.  |  Bhat, MK., et al. 1999. Thyroid. 9: 411-8. PMID: 10319950
2. 17alpha-estradiol-induced VEGF-A expression in rat pituitary tumor cells is mediated through ER independent but PI3K-Akt dependent signaling pathway.  |  Banerjee, S., et al. 2003. Biochem Biophys Res Commun. 300: 209-15. PMID: 12480545
3. The low-density lipoprotein receptor is regulated by estrogen and forms a functional complex with the estrogen-regulated protein ezrin in pituitary GH3 somatolactotropes.  |  Smith, PM., et al. 2004. Endocrinology. 145: 3075-83. PMID: 15044370
4. Phosphorylation of the endogenous thyrotropin-releasing hormone receptor in pituitary GH3 cells and pituitary tissue revealed by phosphosite-specific antibodies.  |  Jones, BW., et al. 2007. J Biol Chem. 282: 12893-906. PMID: 17329249
5. Melatonin-induced calbindin-D9k expression reduces hydrogen peroxide-mediated cell death in rat pituitary GH3 cells.  |  Yoo, YM. and Jeung, EB. 2010. J Pineal Res. 48: 83-93. PMID: 20041988
6. Anacardic acid induces caspase-independent apoptosis and radiosensitizes pituitary adenoma cells.  |  Sukumari-Ramesh, S., et al. 2011. J Neurosurg. 114: 1681-90. PMID: 21275565
7. Role of Neurokinin B and Dynorphin A in pituitary gonadotroph and somatolactotroph cell lines.  |  Mijiddorj, T., et al. 2012. Endocr J. 59: 631-40. PMID: 22641014
8. Regulation of growth hormone secretion by (pro)renin receptor.  |  Tani, Y., et al. 2015. Sci Rep. 5: 10878. PMID: 26039928
9. Lack of Cell Proliferative and Tumorigenic Effects of 4-Hydroxyestradiol in the Anterior Pituitary of Rats: Role of Ultrarapid O-Methylation Catalyzed by Pituitary Membrane-Bound Catechol-O-Methyltransferase.  |  Wang, P., et al. 2017. Chem Res Toxicol. 30: 1448-1462. PMID: 28616971
10. Rundown of GH3 cell K+ conductance response to TRH following patch recording can be obviated with GH3 cell extract.  |  Dufy, B., et al. 1986. Biochem Biophys Res Commun. 137: 388-96. PMID: 3013190
11. Inhibition of mTORC1 by lncRNA H19 via disrupting 4E-BP1/Raptor interaction in pituitary tumours.  |  Wu, ZR., et al. 2018. Nat Commun. 9: 4624. PMID: 30397197
12. Effects of octreotide on autophagy markers and cell viability markers related to metabolic activity in rat pituitary tumor cells.  |  Tulipano, G. and Giustina, A. 2020. Pituitary. 23: 223-231. PMID: 31997055
13. Characterization of an up-stream promoter directing extrapituitary expression of the human prolactin gene.  |  Berwaer, M., et al. 1994. Mol Endocrinol. 8: 635-42. PMID: 8058071