MEG-01 Cell Lysate: sc-2283
- 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
- human whole cell lysate; chronic myelogenous leukemia cells
- whole cell lysate provided as Western blotting positive control
- should be stored at -20°C and repeated freezing and thawing should be minimized
- sample vial should be placed at 95° C for up to 5 minutes, once prior to use
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MEG-01 cell lysate is derived from a human megakaryoblastic leukemia cell line extensively utilized in hematological research to investigate platelet biogenesis and the molecular mechanisms underlying megakaryocyte differentiation. This lysate provides a rich source of cellular proteins and is instrumental in studying signaling pathways such as those mediated by thrombopoietin (TPO), which plays a crucial role in the proliferation and maturation of megakaryocytes. Researchers use MEG-01 cell lysate to explore the activation of downstream signaling cascades, including JAK/STAT, MAPK, and PI3K-Akt pathways, which are pivotal for cell cycle regulation and apoptosis in megakaryocytes. The lysate is also employed in proteomic studies to identify and quantify the expression of transcription factors like GATA-1, FOG-1, and NF-E2, which are essential for platelet gene expression and maturation. By analyzing modifications in protein expression and phosphorylation states, scientists gain insights into the cellular responses to external stimuli, such as cytokines and growth factors, in a controlled research setting. Importantly, this lysate is used to understand fundamental cellular processes and to explain the complex regulation of megakaryocyte physiology, contributing significantly to basic scientific knowledge in hematology.
MEG-01 Cell Lysate References:
- Contribution of leptin receptor N-linked glycans to leptin binding. | Kamikubo, Y., et al. 2008. Biochem J. 410: 595-604. PMID: 17983356
- Proteolysis of plasma-derived factor V following its endocytosis by megakaryocytes forms the platelet-derived factor V/Va pool. | Ayombil, F., et al. 2013. J Thromb Haemost. 11: 1532-9. PMID: 23710903
- Development and characterization of a novel, megakaryocyte NF-κB reporter cell line for investigating inflammatory responses. | Vallance, TM., et al. 2021. J Thromb Haemost. 19: 107-120. PMID: 33037735
- Dengue virus induces interferon-β by activating RNA sensing pathways in megakaryocytes. | Arya, RP., et al. 2021. Immunol Lett. 236: 31-36. PMID: 34111476
- Dengue Virus Dysregulates Master Transcription Factors and PI3K/AKT/mTOR Signaling Pathway in Megakaryocytes. | Lahon, A., et al. 2021. Front Cell Infect Microbiol. 11: 715208. PMID: 34513730
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MEG-01 Cell Lysate | sc-2283 | 500 µg/200 µl | $120.00 |