Saos-2 Cell Lysate: sc-2235

Saos-2 cell lysate is derived from the Saos-2 cell line, an osteosarcoma-derived line used extensively in bone biology and cancer research. This lysate allows for in-depth analysis of the pathways involved in osteoblast differentiation and the molecular mechanisms of bone formation and resorption. Researchers utilize Saos-2 lysate to study the expression and regulatory mechanisms of genes involved in osteogenesis, such as those encoding bone morphogenetic proteins (BMPs) and osteocalcin. It is also used to investigate the role of signaling pathways like Wnt and Hedgehog, which are crucial for bone development and are often aberrantly activated in osteosarcoma. The lysate provides insights into the cell cycle regulation and apoptosis in osteoblastic cells, offering a detailed understanding of the cellular responses to various chemical stimuli or genetic modifications. Additionally, Saos-2 cell lysate helps in exploring the impact of mechanical stress on bone cells, contributing to research on bone density and health. Through these applications, Saos-2 cell lysate aids significantly in advancing the basic science of osteosarcoma and bone biology.

Saos-2 Cell Lysate References:

1. Two related ARID family proteins are alternative subunits of human SWI/SNF complexes.  |  Wang, X., et al. 2004. Biochem J. 383: 319-25. PMID: 15170388
2. Effect of platelet-rich plasma on migration and proliferation of SaOS-2 osteoblasts: role of platelet-derived growth factor and transforming growth factor-beta.  |  Celotti, F., et al. 2006. Wound Repair Regen. 14: 195-202. PMID: 16630109
3. Sp1 family of transcription factors regulates the human alpha2 (XI) collagen gene (COL11A2) in Saos-2 osteoblastic cells.  |  Goto, T., et al. 2006. J Bone Miner Res. 21: 661-73. PMID: 16734381
4. Characterization of a novel mouse monoclonal antibody, clone 1E8.33, highly specific for human procollagen 11A1, a tumor-associated stromal component.  |  García-Ocaηa, M., et al. 2012. Int J Oncol. 40: 1447-54. PMID: 22322826
5. E1A expression might be controlled by miR-214 in cells with low adenovirus productivity.  |  Yanagawa-Matsuda, A., et al. 2012. Virus Res. 170: 85-90. PMID: 22982546
6. The synergistic effect of SaOS-2 cell extract and other bone-inducing agents on human bone cell cultivation.  |  Saif, A., et al. 2012. Drug Discov Ther. 6: 315-20. PMID: 23337819
7. Adsorption according to the Langmuir-Freundlich model is the detection mechanism of the antigen p53 for early diagnosis of cancer.  |  Soares, JC., et al. 2016. Phys Chem Chem Phys. 18: 8412-8. PMID: 26932233
8. In Vitro Generation of Anti-Osteosarcoma Cytotoxic Activity Using Dendritic Cells Loaded with Heat Shock Protein 70-Peptide Complexes.  |  Li, Y., et al. 2019. Fetal Pediatr Pathol. 38: 387-398. PMID: 30955440
9. Hydroxyapatite nanocomposite as a potential agent in osteosarcoma PDT.  |  Elfeky, SA., et al. 2020. Photodiagnosis Photodyn Ther. 32: 102056. PMID: 33068821
10. Sunitinib Exerts In Vitro Immunomodulatory Activity on Sarcomas via Dendritic Cells and Synergizes With PD-1 Blockade.  |  Ocadlikova, D., et al. 2021. Front Immunol. 12: 577766. PMID: 33717062
11. Investigation of anticancer activities of STA-9090 (ganetespib) as a second generation HSP90 inhibitor in Saos-2 osteosarcoma cells.  |  Özgυr, A. 2021. J Chemother. 33: 554-563. PMID: 33794753
12. A rapid, quantitative assay for measuring alkaline phosphatase activity in osteoblastic cells in vitro.  |  Sabokbar, A., et al. 1994. Bone Miner. 27: 57-67. PMID: 7849547
13. Expression of bone morphogenetic proteins by osteoinductive and non-osteoinductive human osteosarcoma cells.  |  Raval, P., et al. 1996. J Dent Res. 75: 1518-23. PMID: 8876605