NIH/3T3 + heat shock Cell Lysate: sc-2217

NIH/3T3 + heat shock cell lysate is derived from NIH/3T3 cells, a mouse embryonic fibroblast cell line, subjected to heat shock treatment. This lysate is extensively used in research to study the cellular stress response, particularly the heat shock response, which is crucial for understanding how cells cope with and recover from stress. Heat shock induces the expression of heat shock proteins (HSPs) that function as molecular chaperones, assisting in the proper folding of proteins and preventing protein aggregation. Researchers employ NIH/3T3 + heat shock cell lysate to investigate the activation of heat shock factor 1 (HSF1), which regulates the transcription of HSP genes. This lysate facilitates the study of key signaling pathways activated during the heat shock response, including the MAPK, PI3K/Akt, and JNK pathways, which are essential for cell survival and apoptosis regulation under stress conditions. It is also used to analyze the post-translational modifications of proteins involved in the stress response, such as phosphorylation and ubiquitination. Additionally, NIH/3T3 + heat shock cell lysate is instrumental in examining the effects of various chemical modulators on the heat shock response, providing insights into the cellular mechanisms of stress adaptation. Recent research has utilized this lysate for proteomic and transcriptomic analyses to identify novel proteins and signaling networks involved in the heat shock response. This lysate remains a valuable resource for advancing our understanding of cellular stress responses and protein homeostasis.