MES-SA/Dx5 Cell Lysate: sc-2284
- 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
- human whole cell lysate; uterine sarcoma cells
- whole cell lysate provided as Western blotting positive control
- should be stored at -20°C and repeated freezing and thawing should be minimized
- sample vial should be placed at 95° C for up to 5 minutes, once prior to use
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MES-SA/Dx5 cell lysate originates from a doxorubicin-resistant uterine sarcoma subline, which serves as a critical tool for studying the mechanisms of chemoresistance in cancer research. This lysate is extensively used to analyze cellular adaptations that confer resistance to chemotherapeutic agents, primarily focusing on alterations in drug efflux mechanisms, DNA repair processes, and apoptotic pathways. Researchers employ the MES-SA/Dx5 lysate to explore the upregulation of ATP-binding cassette (ABC) transporters, particularly those known to actively pump chemotherapeutic drugs out of cells, thereby reducing drug efficacy. By investigating protein expression and post-translational modifications within this cell line, scientists gain insights into the molecular basis of resistance and identify potential targets for overcoming resistance in sarcoma cells. Additionally, this lysate aids in the study of differential gene expression between resistant and non-resistant cell lines, providing a comprehensive understanding of the genetic and epigenetic modifications that occur during the development of resistance. Importantly, the MES-SA/Dx5 lysate is utilized solely for these scientific explorations, focusing on the mechanistic understanding of cellular behavior under stressed conditions. This research contributes significantly to the field of cancer biology by elucidating the complex interactions and survival strategies of cancer cells in the face of chemotherapeutic challenges.
MES-SA/Dx5 Cell Lysate References:
- Cyclosporine, a P-glycoprotein modulator, increases [18F]MPPF uptake in rat brain and peripheral tissues: microPET and ex vivo studies. | Laćan, G., et al. 2008. Eur J Nucl Med Mol Imaging. 35: 2256-66. PMID: 18604533
- Identifying a resistance determinant for the antimitotic natural products disorazole C1 and A1. | Lazo, JS., et al. 2010. J Pharmacol Exp Ther. 332: 906-11. PMID: 20008956
- Water-soluble and cleavable quercetin-amino acid conjugates as safe modulators for P-glycoprotein-based multidrug resistance. | Kim, MK., et al. 2014. J Med Chem. 57: 7216-33. PMID: 25122155
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MES-SA/Dx5 Cell Lysate | sc-2284 | 500 µg/200 µl | $118.00 |